Impact of Glycans on Lipid Membrane Dynamics at the Nanoscale Unveiled by Planar Plasmonic Nanogap Antennas and Atomic Force Spectroscopy
Autor: | Maria F. Garcia-Parajo, Pamina M. Winkler, Marina I. Giannotti, Felix Campelo |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Letter Materials science Lipid Bilayers Force spectroscopy Fluorescence correlation spectroscopy 02 engineering and technology Molecular Dynamics Simulation Compartmentalization (psychology) Microscopy Atomic Force 021001 nanoscience & nanotechnology Glycocalyx 03 medical and health sciences Spectrometry Fluorescence 030104 developmental biology Membrane Biophysics Nanotechnology General Materials Science Hyaluronic Acid Physical and Theoretical Chemistry 0210 nano-technology Lipid bilayer Nanoscopic scale Plasmon |
Zdroj: | The Journal of Physical Chemistry Letters |
ISSN: | 1948-7185 |
DOI: | 10.1021/acs.jpclett.0c03439 |
Popis: | Lateral compartmentalization of the plasma membrane is a prominent feature present at multiple spatiotemporal scales that regulates key cellular functions. The extracellular glycocalyx matrix has recently emerged as an important player that modulates the organization of specific receptors and patterns the lipid bilayer itself. However, experimental limitations in investigating its impact on the membrane nanoscale dynamics have hampered detailed studies. Here, we used photonic nanoantenna arrays combined with fluorescence correlation spectroscopy to investigate the influence of hyaluronic acid (HA), a prominent glycosaminoglycan, on the nanoscale organization of mimetic lipid bilayers. Using atomic force microscopy and force spectroscopy, we further correlated our dynamic measurements with the morphology and mechanical properties of bilayers at the nanoscale. Overall, we find that HA has a profound effect on the dynamics, nanoscale organization, and mechanical properties of lipid bilayers that are enriched in sphingolipids and/or cholesterol, such as those present in living cells. |
Databáze: | OpenAIRE |
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