Synthesis, Self-Assembly and In Vitro Cellular Uptake Kinetics of Nanosized Drug Carriers Based on Aggregates of Amphiphilic Oligomers of N-Vinyl-2-pyrrolidone
| Autor: | Mikhail I. Shtilman, Anna L. Luss, Leonid Gurevich, Oksana Yu. Sizova, Igor A. Kuznetsov, Levi Collin Nelemans, Gunna Christiansen, Cristian Pablo Pennisi, Yaroslav Mezhuev, Pavel P. Kulikov |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
endocrine system Endocytosis Article Wortmannin chemistry.chemical_compound Amphiphile endocytosis General Materials Science Microscopy QC120-168.85 Molecular mass nanocarriers Pinocytosis QH201-278.5 Engineering (General). Civil engineering (General) TK1-9971 chemistry Descriptive and experimental mechanics Drug delivery drug delivery Biophysics Electrical engineering. Electronics. Nuclear engineering Nanocarriers TA1-2040 Drug carrier |
| Zdroj: | Kulikov, P P, Luss, A L, Nelemans, L C, Shtilman, M I, Mezhuev, Y O, Kuznetsov, I A, Sizova, O Y, Christiansen, G, Pennisi, P & Gurevich, L 2021, ' Synthesis, Self-Assembly and In Vitro Cellular Uptake Kinetics of Nanosized Drug Carriers Based on Aggregates of Amphiphilic Oligomers of N-Vinyl-2-pyrrolidone ', Materials, vol. 14, no. 20, 5977 . https://doi.org/10.3390/ma14205977 Materials Volume 14 Issue 20 Materials, Vol 14, Iss 5977, p 5977 (2021) |
| DOI: | 10.3390/ma14205977 |
| Popis: | Development of nanocarrier-based drug delivery systems is a major breakthrough in pharmacology, promising targeted delivery and reduction in drug toxicity. On the cellular level, encapsulation of a drug substantially affects the endocytic processes due to nanocarrier–membrane interaction. In this study we synthesized and characterized nanocarriers assembled from amphiphilic oligomers of N-vinyl-2-pyrrolidone with a terminal thiooctadecyl group (PVP-OD). It was found that the dissolution free energy of PVP-OD depends linearly on the molecular mass of its hydrophilic part up to M¯n = 2 × 104, leading to an exponential dependence of critical aggregation concentration (CAC) on the molar mass. A model hydrophobic compound (DiI dye) was loaded into the nanocarriers and exhibited slow release into the aqueous phase on a scale of 18 h. Cellular uptake of the loaded nanocarriers and that of free DiI were compared in vitro using glioblastoma (U87) and fibroblast (CRL2429) cells. While the uptake of both DiI/PVP-OD nanocarriers and free DiI was inhibited by dynasore, indicating a dynamin-dependent endocytic pathway as a major mechanism, a decrease in the uptake rate of free DiI was observed in the presence of wortmannin. This suggests that while macropinocytosis plays a role in the uptake of low-molecular components, this pathway might be circumvented by incorporation of DiI into nanocarriers. |
| Databáze: | OpenAIRE |
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