Fibroblast Growth Factor Release by Bovine Endothelial Cells and Human Astrocytoma Cells in Culture is Density Dependent
| Autor: | Henry G. Friesen, Yuji Sato, Reiko Sato, Paul R. Murphy |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Cell type medicine.medical_treatment Blotting Western Basic fibroblast growth factor Cell Count Astrocytoma Biology Fibroblast growth factor chemistry.chemical_compound Endocrinology Internal medicine Tumor Cells Cultured medicine Animals Humans Receptor Molecular Biology Cells Cultured Immunoassay Cell growth Growth factor Endothelium Corneal General Medicine Blotting Northern Molecular biology Culture Media Fibroblast Growth Factors Endothelial stem cell chemistry Cell culture Cattle |
| Zdroj: | Molecular Endocrinology. 3:744-748 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | Basic fibroblast growth factor (bFGF) is a potent endothelial cell mitogen whose actions are mediated by binding to specific cell surface receptors on a variety of cell types. However, the amino acid sequence of bFGF does not contain a classical signal peptide sequence and the extent to which cellular stores of this mitogen are released is still a matter of some controversy. In the present study we examined the release of immunoreactive bFGF into serum-free conditioned medium of bovine corneal endothelial cells (BCE) and a human astrocytoma cell line, U87-MG. Western blotting analysis of BCE conditioned medium using N-terminal specific anti-bFGF serum revealed a single immunoreactive band of 32 kilodaltons, which was reduced to 18 kilodaltons in the presence of 8 M urea. Using a sensitive two-site immunoradiometric assay we were able to quantify the release of immunoreactive bFGF into the culture medium by BCE cells and by the human astrocytoma cell line U87-MG. In each case the release of bFGF was cell density dependent, but under all conditions the level of bFGF released was significantly greater in the transformed astrocytoma line, ranging from 15- to 50-fold higher than in the BCE cultures under various conditions. At 30% confluence the concentration of immunoreactive bFGF in the medium was maintained at a constant level for up to 24 h. However, the level of immunoreactive bFGF declined rapidly in confluent cells.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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