Cells Derived from the Coelomic Epithelium Contribute to Multiple Gastrointestinal Tissues in Mouse Embryos
| Autor: | Joaquín Gaztambide, Rita Carmona, Ramón Muñoz-Chápuli, Andrea Mattiotti, Elena Cano |
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| Přispěvatelé: | [Carmona,R, Cano,E, Mattiotti,A, Muñoz-Chápuli,R] Department of Animal Biology, Faculty of Science, University of Málaga, Málaga, Spain. [Gaztambide,J] Pediatric Surgery Unit. Maternity and Children Hospital, Regional Hospital Carlos Haya, Málaga, Spain., This study was supported by grants BFU2008-02384, BFU2011-25304 (Ministerio de Ciencia e Innovación), RD06/0010/0015 (TerCel network, ISCIII), P08-CTS-03618 and P11-CTS-7564 (Junta de Andalucía). |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation [Medical Subject Headings]
Pathology Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Membrane Proteins::Membrane Transport Proteins::Ion Channels::Chloride Channels [Medical Subject Headings] Embryology Células epiteliales Anatomy::Embryonic Structures::Germ Layers::Mesoderm [Medical Subject Headings] Anatomy and Physiology Receptor Platelet-Derived Growth Factor alpha Mouse Cellular differentiation Organogenesis lcsh:Medicine Cell Fate Determination Ratones transgénicos Digestive Anatomy Mesoderm Mice Organisms::Eukaryota::Animals [Medical Subject Headings] Morphogenesis Organisms::Eukaryota::Animals::Animal Population Groups::Animals Genetically Modified::Mice Transgenic [Medical Subject Headings] Intestinal Mucosa lcsh:Science Multidisciplinary Cell Differentiation Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Membrane Proteins::Receptors Cell Surface::Receptors Peptide::Receptors Growth Factor::Receptors Platelet-Derived Growth Factor [Medical Subject Headings] Animal Models Coelomic epithelium Cell biology Intestines medicine.anatomical_structure Diferenciación Celular symbols Linaje celular Anatomy::Digestive System::Gastrointestinal Tract::Intestines [Medical Subject Headings] Factor 9 de crecimiento de fibroblastos Research Article Fibroblast Growth Factor 9 medicine.medical_specialty Mesenchyme Mice Transgenic Cell Migration Biology Receptor alfa de factor de crecimiento derivados de plaquetas symbols.namesake Músculo liso Model Organisms Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Fibroblast Growth Factors::Fibroblast Growth Factor 9 [Medical Subject Headings] Chloride Channels medicine Animals Cell Lineage Anoctamin-1 Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] Mesenchymal stem cell lcsh:R fungi Epithelial Cells Muscle Smooth Intestinos Embryonic stem cell Epithelium Phenomena and Processes::Cell Physiological Phenomena::Cell Lineage [Medical Subject Headings] Interstitial cell of Cajal Anatomy::Cells::Epithelial Cells [Medical Subject Headings] Anatomy::Tissues::Muscles::Muscle Smooth [Medical Subject Headings] lcsh:Q Canales del cloro Digestive System Organism Development Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 2, p e55890 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Journal Article; Research Support, Non-U.S. Gov't; Gut mesodermal tissues originate from the splanchnopleural mesenchyme. However, the embryonic gastrointestinal coelomic epithelium gives rise to mesenchymal cells, whose significance and fate are little known. Our aim was to investigate the contribution of coelomic epithelium-derived cells to the intestinal development. We have used the transgenic mouse model mWt1/IRES/GFP-Cre (Wt1(cre)) crossed with the Rosa26R-EYFP reporter mouse. In the gastrointestinal duct Wt1, the Wilms' tumor suppressor gene, is specific and dynamically expressed in the coelomic epithelium. In the embryos obtained from the crossbreeding, the Wt1-expressing cell lineage produces the yellow fluorescent protein (YFP) allowing for colocalization with differentiation markers through confocal microscopy and flow cytometry. Wt1(cre-YFP) cells were very abundant throughout the intestine during midgestation, declining in neonates. Wt1(cre-YFP) cells were also transiently observed within the mucosa, being apparently released into the intestinal lumen. YFP was detected in cells contributing to intestinal vascularization (endothelium, pericytes and smooth muscle), visceral musculature (circular, longitudinal and submucosal) as well as in Cajal and Cajal-like interstitial cells. Wt1(cre-YFP) mesenchymal cells expressed FGF9, a critical growth factor for intestinal development, as well as PDGFRα, mainly within developing villi. Thus, a cell population derived from the coelomic epithelium incorporates to the gut mesenchyme and contribute to a variety of intestinal tissues, probably playing also a signaling role. Our results support the origin of interstitial cells of Cajal and visceral circular muscle from a common progenitor expressing anoctamin-1 and SMCα-actin. Coelomic-derived cells contribute to the differentiation of at least a part of the interstitial cells of Cajal. Yes |
| Databáze: | OpenAIRE |
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