Blockade of the M1 muscarinic acetylcholine receptors impairs eyeblink serial feature-positive discrimination learning in mice
| Autor: | Shandhya DebNath, Norifumi Tanaka, Ashrafur Rahman, Shigenori Kawahara, Nuruzzaman |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Social Sciences Biochemistry Hippocampus Discrimination Learning Mice Learning and Memory Cognition 0302 clinical medicine Muscarinic acetylcholine receptor Medicine and Health Sciences Psychology Medicine Discrimination learning Receptor Sulfonamides Multidisciplinary Brain Muscarinic acetylcholine receptor M1 Conditioning Eyelid Bioassays and Physiological Analysis Eyeblink conditioning Sensory Perception Anatomy Muscle Electrophysiology Research Article Signal Transduction Transmembrane Receptors Science Stimulus (physiology) Research and Analysis Methods 03 medical and health sciences Sensory Cues Memory Thiadiazoles Animals Learning Cued speech Behavior Blinking Electromyography business.industry Receptor Muscarinic M1 Electrophysiological Techniques Cognitive Psychology Biology and Life Sciences Proteins Classical conditioning Cell Biology Mice Inbred C57BL 030104 developmental biology Acetylcholine Receptors Muscarinic Acetylcholine Receptors Cognitive Science Conditioned Response Perception business Neuroscience Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 15, Iss 8, p e0237451 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0237451 |
| Popis: | The serial feature-positive discrimination task requires the subjects to respond differentially to the identical stimulus depending on the temporal context given by a preceding cue stimulus. In the present study, we examined the involvement of the M1 muscarinic acetylcholine receptors using a selective M1 antagonist VU0255035 in the serial feature-positive discrimination task of eyeblink conditioning in mice. In this task, mice received a 2-s light stimulus as the conditional cue 5 or 6 s before the presentation of a 350-ms tone conditioned stimulus (CS) paired with a 100-ms peri-orbital electrical shock (cued trials), while they did not receive the cue before the presentation of the CS alone (non-cued trials). Each day mice randomly received 30 cued and 30 non-cued trials. We found that VU0255035 impaired acquisition of the conditional discrimination as well as the overall acquisition of the conditioned response (CR) and diminished the difference in onset latency of the CR between the cued and non-cued trials. VU0255035 administration to the control mice after sufficient learning did not impair the pre-acquired conditional discrimination or the CR expression itself. These effects of VU0255035 were almost similar to those with the scopolamine in our previous study, suggesting that among the several types of muscarinic acetylcholine receptors, the M1 receptors may play an important role in the acquisition of the conditional discrimination memory but not in mediating the discrimination itself after the memory had formed in the eyeblink serial feature-positive discrimination learning. |
| Databáze: | OpenAIRE |
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