Hepatoprotective effects of rice-derived peptides against acetaminophen-induced damage in mice
| Autor: | Etsuko Suzaki, Misugi Uraji, Kayoko Kawakami, Koji Kawakami, Seiji Tsuboi, Chie Moritani, Tadashi Hatanaka, Akiko Fujita |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Clinical Biochemistry Intraperitoneal injection Medicine (miscellaneous) Pharmacology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactate dehydrogenase medicine glutathione acetaminophen chemistry.chemical_classification Reactive oxygen species Nutrition and Dietetics rice-derived peptide Centrilobular necrosis digestive oral and skin physiology food and beverages Glutathione digestive system diseases Acetaminophen stomatognathic diseases 030104 developmental biology chemistry Biochemistry 030220 oncology & carcinogenesis Original Article Oxidative stress liver injury medicine.drug |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| DOI: | 10.3164/jcbn.16-44 |
| Popis: | Glutathione, the most abundant intracellular antioxidant, protects cells against reactive oxygen species induced oxidative stress and regulates intracellular redox status. We found that rice peptides increased intracellular glutathione levels in human hepatoblastoma HepG2 cells. Acetaminophen is a commonly used analgesic. However, an overdose of acetaminophen causes severe hepatotoxicity via depletion of hepatic glutathione. Here, we investigated the protective effects of rice peptides on acetaminophen-induced hepatotoxicity in mice. ICR mice were orally administered rice peptides (0, 100 or 500 mg/kg) for seven days, followed by the induction of hepatotoxicity via intraperitoneal injection of acetaminophen (700 mg/kg). Pretreatment with rice peptides significantly prevented increases in serum alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase levels and protected against hepatic glutathione depletion. The expression of γ-glutamylcysteine synthetase, a key regulatory enzyme in the synthesis of glutathione, was decreased by treatment with acetaminophen, albeit rice peptides treatment recovered its expression compared to that achieved treatment with acetaminophen. In addition, histopathological evaluation of the livers also revealed that rice peptides prevented acetaminophen-induced centrilobular necrosis. These results suggest that rice peptides increased intracellular glutathione levels and could protect against acetaminophen-induced hepatotoxicity in mice. |
| Databáze: | OpenAIRE |
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