Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer
| Autor: | Xiong‑Bing Zu, Huihui Zhang, Fan Qi, You‑Han Cao, Min‑Feng Chen |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Oncology Letters. 10:2610-2616 |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | The present study aimed to explore the expression and clinical significance of microRNA-21 (miR-21), maspin and vascular endothelial growth factor C (VEGF-C) in bladder cancer (BC). A total of 53 BC samples and 12 normal bladder tissue samples were collected. Total messenger RNA (mRNA) was extracted, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression of miR-21 and maspin in BC and normal bladder tissues. Immunohistochemistry was used for the detection of maspin and VEGF-C protein expression. Furthermore, the correlations between these molecules and certain clinicopathological parameters were investigated, and survival analysis was performed to assess their prognostic significance. miR-21 mRNA expression and VEGF-C protein expression were increased in BC tissues compared with those in normal bladder tissues, whereas maspin mRNA and protein expression levels in BC tissues were significantly decreased (P |
| Databáze: | OpenAIRE |
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