Effect of bovine milk fat-based infant formulae on microbiota, metabolites and stool parameters in healthy term infants in a randomized, crossover, placebo-controlled trial
| Autor: | Looijesteijn, Ellen, Brouwer, Rutger W W, Schoemaker, Ruud J W, Ulfman, Laurien H, Ham, Stephanie L, Jeurink, Prescilla, Karaglani, Eva, van IJcken, Wilfred F J, Manios, Yannis, Afd Pharmacology, Pharmacology |
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| Přispěvatelé: | Cell biology, Afd Pharmacology, Pharmacology |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Faecal fatty acid soaps
Nutrition and Dietetics Deep sequencing Stool consistency Milk fat SN-2-palmitate Endocrinology Diabetes and Metabolism Calcium excretion Faecal metabolites Microbiota digestive oral and skin physiology Environmental and Occupational Health Public Health Environmental and Occupational Health Medicine (miscellaneous) Diabetes and Metabolism fluids and secretions Endocrinology SDG 3 - Good Health and Well-being Public Health |
| Zdroj: | BMC Nutrition, 8(1):93. BioMed Central Ltd. BMC Nutrition, 8(1), 1. BioMed Central |
| ISSN: | 2055-0928 |
| Popis: | Background Natural enrichment of sn-2 palmitate content of infant formulae by using bovine milk fat is known to reduce formation of faecal fatty acid soaps and to improve stool consistency, but effects on gut microbiota composition are unknown. The purpose of this study was to test the influence of milk fat-based formula high in sn-2 palmitate on the infants’ gut microbiota composition and to confirm the beneficial effects of the formula on formation of faecal fatty acid soaps and stool consistency. Methods Twenty-two healthy term, formula-fed infants were enrolled in a single-blinded randomized, crossover, placebo-controlled trial. After a 2-week run-in period, infants received either a 50% milk fat-based formula containing 39% sn-2 palmitate (MF) or a vegetable fat-based formula (VF) containing 10% sn-2 palmitate in a 2 × 2-week crossover design. Faecal microbiota composition was the primary outcome of the study. Other outcomes included faecal fatty acid soap excretion, calcium excretion, gut comfort parameters and faecal metabolites. Results Microbiota analysis showed that bifidobacteria dominated the gut microbiota of most infants. Neither alpha- nor beta-diversity was significantly influenced by the intervention. Also, abundance of metabolic pathways was independent of the intervention. The MF formula resulted in significantly lower faecal levels of palmitic acid soap (p = 0.0002) and total fatty acid soaps (p = 0.0001) than the VF formula. Additionally, calcium excretion and palmitic acid concentration were significantly (p = 0.0335) lower in stool samples after MF intervention. Furthermore, a significant physiological effect on softer stools was observed in the MF intervention compared to the VF intervention (p = 0.02). Of the 870 measured faecal metabolites, 190 were significantly different after MF and VF intervention (FDR corrected p Conclusions Replacing part of the vegetable fat in infant formula with bovine milk fat with high sn-2 palmitate levels did not change the microbiota composition, although a reduction in faecal palmitate soaps, total fatty acid soaps and calcium excretion while improving stool consistency in the MF intervention was confirmed. In addition, 190 faecal metabolites were significantly different, many related to the fat source. Trial registration Netherlands Trial Registry Identifier: NL7815 19/06/2019. |
| Databáze: | OpenAIRE |
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