Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection
| Autor: | Afroditi Petropoulou, Stergios Pispas, Efrosyni Themistou, Christos Riziotis, Thomas J. Gibson |
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| Rok vydání: | 2018 |
| Předmět: |
Polymers
02 engineering and technology Methacrylate 01 natural sciences Gel permeation chromatography chemistry.chemical_compound Materials Science(all) Amphiphile Polymer chemistry Copolymer Optical fibers General Materials Science Bovine serum albumin Methyl methacrylate biology Biosensing Chemistry 010401 analytical chemistry Cationic polymerization Proteins 021001 nanoscience & nanotechnology Condensed Matter Physics Block copolymers 0104 chemical sciences Photonic sensors biology.protein Chemical binding 0210 nano-technology |
| Zdroj: | Petropoulou, A, Gibson, T J, Themistou, E, Pispas, S & Riziotis, C 2018, ' Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection ', Materials Chemistry and Physics, vol. 216, pp. 421-428 . https://doi.org/10.1016/j.matchemphys.2018.06.027 |
| ISSN: | 0254-0584 |
| Popis: | Novel amphiphilic block copolymers having both hydrophobic poly(methyl methacrylate) (PMMA) and hydrophilic poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) blocks have been designed and synthesized for efficient protein detection in photonic-based sensing. Both the cationic PMMA117-b-PDMAEMA16 and the cationic vinyl-sulfone functionalized PMMA117-b-P(DMAEMA17-VSTEMA2) block copolymers, where VSTEMA is 2-(2-(vinylsulfonyl)ethylthio)ethyl methacrylate, were synthesized from a water insoluble hydrophobic PMMA block, which facilitated the formation of stable overlayers on the silica optical fibers surface. The well-defined structure of the co-polymers was confirmed by gel permeation chromatography (GPC). The presence of the cationic PDMAEMA block and the vinyl-sulfone double bonds led to reversible electrostatic binding of negatively charged proteins like bovine serum albumin (BSA) and non-reversible chemical binding by thiol-ene reactions with cysteine in proteins, respectively. The sensing properties of these materials were assessed and confirmed by ATR-FTIR analysis and by the characterization of fabricated sensing heads on silica optical fibers functionalized with suitably deposited overlayers. The sensing assessment revealed the requirements for deposited overlayer characteristics towards proteins' detection sensitivity and selectivity enhancement. |
| Databáze: | OpenAIRE |
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