Effects of di(n-butyl) and monobutyl phthalate on steroidogenesis pathways in the murine Leydig tumor cell line MLTC-1
| Autor: | Ling Leng, Xu Chen, Xi Chen, Qing-hong Zhou, Naijun Tang, Zengrong Sun |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
endocrine system medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases Time Factors Cytochrome Cell Survival medicine.drug_class Health Toxicology and Mutagenesis Phthalic Acids Toxicology Gene Expression Regulation Enzymologic Mice chemistry.chemical_compound Testicular Neoplasms Cell Line Tumor Internal medicine medicine Animals Insulin Testosterone Cholesterol Side-Chain Cleavage Enzyme RNA Messenger Active metabolite Pharmacology chemistry.chemical_classification Dose-Response Relationship Drug biology Chemistry Cholesterol side-chain cleavage enzyme Phthalate Proteins General Medicine Androgen Dibutyl Phthalate In vitro Gene Expression Regulation Neoplastic Endocrinology Enzyme biology.protein Leydig Cell Tumor circulatory and respiratory physiology Hormone |
| Zdroj: | Environmental Toxicology and Pharmacology. 36:332-338 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/j.etap.2013.04.013 |
| Popis: | Di(n-butyl) phthalate (DBP) and its active metabolite monobutyl phthalate (MBP) have been shown to disrupt reproductive organ growth. The objective of this study was to evaluate the effects of DBP/MBP on steroidogenesis in the murine Leydig tumor cell line MLTC-1 in vitro. MLTC-1 cells were incubated with various concentrations of DBP (100, 1, 0.01, and 0 μmol/l in DMSO) and MBP (1000, 10, 0.1, and 0 μmol/l in DMSO) for 24 h. Testosterone secretion was stimulated at the lowest doses and inhibited at higher treatment doses of DBP and MBP. The mRNA levels of the side-chain cleavage enzyme (P450scc), cytochrome p450c17 (P450c17) and 3β-hydroxy-steroid dehydrogenase (3βHSD) were significantly reduced in the phthalate-exposed groups, whereas, the transcription and translation of insulin-like hormone 3 (INSL3) was affected by DBP and MBP. Alterations of the steroidogenic enzymes and INSL3 in MLTC-1 cells may be involved in the biphasic effects of DBP/MBP on androgen production. |
| Databáze: | OpenAIRE |
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