A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions
| Autor: | Daniel M. Joel, Koichi Yoneyama, Chaitali Bhattacharya, Yoram Kapulnik, Smadar Wininger, Joseph Hershenhorn, Einav Mayzlish-Gati, Hinanit Koltai, Evgenya Dor, Kaori Yoneyama, Nathalie Resnick, Sivarama P. LekKala |
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| Rok vydání: | 2010 |
| Předmět: |
Physiology
Parasitic plant Mutant mycorrhiza Strigolactone strigolactones Plant Science tomato Lycopersicon Lactones Solanum lycopersicum Botany shoot branching Plant Proteins biology fungi Wild type food and beverages Plants Genetically Modified CCD8 biology.organism_classification Research Papers CCD7 grafted plants Lateral shoot Shoot Rootstock Plant Shoots |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in tomato (Solanum lycopersicon) of strigolactone deficiency is presented here. Sl-ORT1, previously identified as resistant to the parasitic plant Orobanche, had lower levels of arbuscular mycorrhizal fungus (Glomus intraradices) colonization, possibly as a result of its reduced ability to induce mycorrhizal hyphal branching. Biochemical analysis of mutant root extracts suggested that it produces only minute amounts of two of the tomato strigolactones: solanacol and didehydro-orobanchol. Accordingly, the transcription level of a key enzyme (CCD7) putatively involved in strigolactone synthesis in tomato was reduced in Sl-ORT1 compared with the wild type (WT). Sl-ORT1 shoots exhibited increased lateral shoot branching, whereas exogenous application of the synthetic strigolactone GR24 to the mutant restored the WT phenotype by reducing the number of lateral branches. Reduced lateral shoot branching was also evident in grafted plants which included a WT interstock, which was grafted between the mutant rootstock and the scion. In roots of these grafted plants, the CCD7 transcription level was not significantly induced, nor was mycorrhizal sensitivity restored. Hence, WT-interstock grafting, which restores mutant shoot morphology to WT, does not restore mutant root properties to WT. Characterization of the first tomato strigolactone-deficient mutant supports the putative general role of strigolactones as messengers of suppression of lateral shoot branching in a diversity of plant species. |
| Databáze: | OpenAIRE |
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