Combination adjuvants for the induction of potent, long-lasting antibody and T-cell responses to influenza vaccine in mice

Autor: Mildred Ugozzoli, Simona Tavarini, Sandra Nuti, Manmohan Singh, Emanuele Montomoli, Barbara Baudner, Hanno Scheffczik, Jina Kazzaz, Rino Rappuoli, Andreas Wack, Ilaria Manini, Derek T. O'Hagan, Giuseppe Del Giudice, Anne Katrin Hilbert
Rok vydání: 2008
Předmět:
Calcium Phosphates
Cellular immunity
Polymers
medicine.medical_treatment
MF59
Polysorbates
Antibodies
Viral
medicine.disease_cause
Adjuvants
Influenza vaccine
T-cell cytokine response
Mice
Influenza A Virus
H1N1 Subtype
Polylactic Acid-Polyglycolic Acid Copolymer
Antibody Specificity
Influenza A virus
Mice
Inbred BALB C
education.field_of_study
Orthomyxoviridae
Vaccination
Infectious Diseases
Influenza Vaccines
Vaccines
Subunit
Molecular Medicine
Emulsions
Female
Adjuvant
Squalene
Population
Biology
Injections
Intramuscular
Cell Line
Interferon-gamma
Adjuvants
Immunologic
Orthomyxoviridae Infections
Neutralization Tests
medicine
Animals
Lactic Acid
education
General Veterinary
General Immunology and Microbiology
Influenza A Virus
H3N2 Subtype
Public Health
Environmental and Occupational Health
Th1 Cells
Virology
Influenza A virus subtype H5N1
Influenza B virus
Immunoglobulin G
Immunology
Polyglycolic Acid
Spleen
Zdroj: Vaccine. 26:552-561
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2007.11.054
Popis: Influenza is controlled by protective titres of neutralizing antibodies, induced with the help of CD4 T-cells, and by antiviral T-cell effector function. Adjuvants are essential for the efficient vaccination of a naïve population against avian influenza. We evaluated a range of adjuvants for their ability to enhance, in naïve mice, protective hemagglutination inhibition (HI) titres, which represent the generally accepted correlate of protection, virus-neutralizing titres and T-cell responses to a new generation influenza vaccine produced in cell culture. The selected adjuvants include alum, calcium phosphate (CAP), MF59, the delivery system poly-(lactide co-glycolide) (PLG) and the immune potentiator CpG. MF59 was clearly the most potent single adjuvant and induced significantly enhanced, long-lasting HI and neutralizing titres and T-cell responses in comparison to all alternatives. The combination of alum, MF59, CAP or PLG with CpG generally induced slightly more potent titres. The addition of CpG to MF59 also induced a more potent Th1 cellular immune response, represented by higher IgG2a titres and the induction of a strongly enhanced IFN-gamma response in splenocytes from immunized mice. These observations have significant implications for the development of new and improved flu vaccines against pandemic and inter-pandemic influenza virus strains.
Databáze: OpenAIRE
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