The glycoproteins EDIL3 and MFGE8 regulate vesicle - mediated eggshell calcification in a new model for avian biomineralisation
| Autor: | Joël Gautron, Maxwell T. Hincke, Lilian Stapane, Nathalie Le Roy |
|---|---|
| Přispěvatelé: | Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA), University of Ottawa [Ottawa], Gautron, Joël, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biomineralization Proteomics Proteome [SDV]Life Sciences [q-bio] Glycobiology and Extracellular Matrices Biochemistry chemistry.chemical_compound Egg Shell Eggshell ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Vesicle biominéralisation Milk Proteins Amorphous calcium carbonate Cell biology Antigens Surface Female chemistry.chemical_element coquille d'oeuf Calcium Calcium Carbonate 03 medical and health sciences Calcification Physiologic medicine Animals Humans [INFO]Computer Science [cs] Shell calcification Molecular Biology Glycoproteins glycoprotéine [SDV.GEN]Life Sciences [q-bio]/Genetics 030102 biochemistry & molecular biology Calcium-Binding Proteins Uterus Cell Biology Lipid Droplets poule pondeuse medicine.disease [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics 030104 developmental biology chemistry Gene Expression Regulation Glycolipids Glycoprotein Cell Adhesion Molecules Chickens absorption du calcium Calcification |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2019, 294 (40), pp.14526-14545. ⟨10.1074/jbc.RA119.009799⟩ J Biol Chem Journal of Biological Chemistry 40 (294), 14526-14545. (2019) |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.RA119.009799⟩ |
| Popis: | The avian eggshell is a critical physical barrier, which permits extra-uterine development of the embryo. Its formation involves the fastest known biomineralization process in vertebrates. The eggshell consists of proteins and proteoglycans that interact with the mineral phase to impart its specific microstructure and mechanical properties. In this study, we investigated the role of epidermal growth factor (EGF)-like repeats and discoidin-like domains 3 (EDIL3) and milk fat globule–EGF factor 8 (MFGE8), two glycoproteins that are consistently detected in eggshell proteomes. We verified their common evolutionary history and identified the timing of the duplication event giving rise to these two distinct proteins. Edil3/mfge8 chromosomal locations revealed a nested syntenous relationship with other genes (hapln1/hapln3 and vcan/acan) that are also involved in vertebrate calcification. EDIL3 and MFGE8 proteins possess EGF-like and coagulation factor 5/8 (F5/8C) domains, and their 3D structures predicted that they bind calcium and extracellular vesicles. In chicken, we confirmed the presence of EDIL3 and MFGE8 proteins in eggshell, uterine fluid, and uterus. We observed that only edil3 is overexpressed in tissues in which eggshell mineralization takes place and that this overexpression occurs only at the onset of shell calcification. We therefore propose a model in which EDIL3 and, to a lesser extent, MFGE8 proteins guide vesicles containing amorphous calcium carbonate to the mineralization site. This model was supported by the observation that extracellular vesicles accumulate in uterine fluid during eggshell calcification and that they contain high levels of calcium, carbon, and oxygen that correspond to calcium carbonate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|