Influences of high-voltage electrical burns on the pulmonary microcirculation in rabbits
| Autor: | Qingfu Zhang, Bing Xie, Hong-bo Shao, Che-jiang Wang, L. Wang, J K Feng, Shunjiang Xu, Yong-qiang Bai, Huimin Zhou |
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| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Physiology Capillary endothelial cells Microcirculation 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Physiology (medical) Pressure medicine Animals 030212 general & internal medicine Lung Inflammation Hemostasis Pulmonary Gas Exchange Chemistry Endoplasmic reticulum Burns Electric Degranulation Thrombosis 030208 emergency & critical care medicine Lung Injury Hematology Laser Doppler velocimetry Pulmonary microcirculation Capillaries Oxygen Pulmonary Alveoli medicine.anatomical_structure Models Animal Endoplasmic Reticulum Rough Rabbits Blood Gas Analysis Cardiology and Cardiovascular Medicine Perfusion |
| Zdroj: | Clinical Hemorheology and Microcirculation. 62:193-203 |
| ISSN: | 1875-8622 1386-0291 |
| DOI: | 10.3233/ch-141921 |
| Popis: | This study was performed to investigate the effects of high-voltage electrical burns (HEB) on the pulmonary microcirculation in rabbits. Total of 120 rabbits were randomly divided into control and HEB group using a random number table. HEB model was developed with a voltage regulator and experimental transformer. Laser Doppler perfusion imager was utilized to monitor and quantify the blood perfusion in pulmonary microcirculation. The microvascular morphologic changes of the lung were observed using light microscopy and transmission electron microscope (TEM). The lung wet/dry weight ratio and the PaO2 were determined. The values of blood perfusion in rabbit pulmonary microcirculation in the HEB group were decreased at 5 min, but increased at 1 h after burn (P < 0.01) and then decreased gradually. Light microscopy reveals microthrombus formation in pulmonary venules and bleeding in venous capillaries in HEB group. We found the number of microvilli in the capillary endothelial cells decreased, the rough endoplasmic reticulum expanded and severe degranulation occurred, the mitochondrial cristae fused or disappeared, and severe edema surrounded the capillary endothelial cells by TEM. The values of lung wet/dry weight ratio were higher and the PaO2 were lower than that of before burn group (P < 0.01). These results demonstrated that microcirculatory disorders play a major role in the development of progressive lung damage after high-voltage electrical burns. |
| Databáze: | OpenAIRE |
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