Comparative Analysis of Bacterial Community Composition and Structure in Clinically Symptomatic and Asymptomatic Central Venous Catheters

Autor: Bruno François, Marie-Cécile Ploy, Elodie Couvé-Deacon, Christophe Beloin, Jean-Marc Ghigo, Irène Kriegel, Ashwini Chauhan, Delphine Chainier, Aimee K. Wessel, Marie-Christine Escande, Franziska A. Stressmann, Sylvaine Durand-Fontanier
Přispěvatelé: SITBON, Pascale, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Génétique des Biofilms, Institut Pasteur [Paris] (IP), Service de Bactériologie, Virologie, Hygiène [CHU Limoges], CHU Limoges, Service de Chirurgie digestive, endocrinienne et générale [CHU Limoges], Institut Curie [Paris], Centre d'Investigation Clinique de Limoges (CIC1435), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM), F.A.S. was a Marie Curie Fellow, and this work was supported by grants from the Institut Pasteur and Institut Pasteur-Institut Curie PTR/PIC 'Nosocomial Infections program,' the Institut Pasteur-Institut Mérieux Collaborative Research program, the French Government’s Investissement d’avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant ANR-10-LABX-62-IBEID), and the 'Fondation Pour la Recherche Médicale' grant (Equipe FRM DEQ20140329508)., We thank Julie Vignaud and Roselyne Droual for their assistance during catheter collection., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris], Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Limoges
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
Pathology
030106 microbiology
lcsh:QR1-502
Biology
medicine.disease_cause
Microbiology
Asymptomatic
bacterial community
lcsh:Microbiology
biofilm
Clinical Science and Epidemiology
03 medical and health sciences
0302 clinical medicine
Bacterial colonization
Microbial ecology
Internal medicine
medicine
Colonization
030212 general & internal medicine
Molecular Biology
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Risk of infection
Pathogenic bacteria
Bacteria Present
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
QR1-502
3. Good health
Community composition
catheter colonization
medicine.symptom
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
ecology
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Research Article
Zdroj: mSphere
mSphere, Vol 2, Iss 5, p e00146-17 (2017)
MSphere
MSphere, 2017, 2 (5), pp.00146-17 ⟨10.1128/mSphere.00146-17⟩
mSphere, Vol 2, Iss 5 (2017)
MSphere, American Society for Microbiology., 2017, 2 (5), pp.00146-17 ⟨10.1128/mSphere.00146-17⟩
ISSN: 2379-5042
Popis: Totally implanted venous access ports (TIVAPs) are commonly used implants for the management of acute or chronic pathologies. Although their use improves the patient’s health care and quality of life, they are associated with a risk of infection and subsequent clinical complications, often leading to implant removal. While all TIVAPs appear to be colonized, only a fraction become infected, and the relationship between nonpathogenic organisms colonizing TIVAPs and the potential risk of infection is unknown. We explored bacteria present on TIVAPs implanted in patients with or without signs of TIVAP infection and identified differences in phylum composition and community structure. Our data suggest that the microbial ecology of intravascular devices could be predictive of TIVAP infection status and that ultimately a microbial ecological signature could be identified as a tool to predict TIVAP infection susceptibility and improve clinical management.
Totally implanted venous access ports (TIVAPs) are commonly used catheters for the management of acute or chronic pathologies. Although these devices improve health care, repeated use of this type of device for venous access over long periods of time is also associated with risk of colonization and infection by pathogenic bacteria, often originating from skin. However, although the skin microbiota is composed of both pathogenic and nonpathogenic bacteria, the extent and the consequences of TIVAP colonization by nonpathogenic bacteria have rarely been studied. Here, we used culture-dependent and 16S rRNA gene-based culture-independent approaches to identify differences in bacterial colonization of TIVAPs obtained from two French hospitals. To explore the relationships between nonpathogenic organisms colonizing TIVAPs and the potential risk of infection, we analyzed the bacterial community parameters between TIVAPs suspected (symptomatic) or not (asymptomatic) of infection. Although we did not find a particular species assemblage or community marker to distinguish infection risk on an individual sample level, we identified differences in bacterial community composition, diversity, and structure between clinically symptomatic and asymptomatic TIVAPs that could be explored further. This study therefore provides a new view of bacterial communities and colonization patterns in intravascular TIVAPs and suggests that microbial ecology approaches could improve our understanding of device-associated infections and could be a prognostic tool to monitor the evolution of bacterial communities in implants and their potential susceptibility to infections. IMPORTANCE Totally implanted venous access ports (TIVAPs) are commonly used implants for the management of acute or chronic pathologies. Although their use improves the patient’s health care and quality of life, they are associated with a risk of infection and subsequent clinical complications, often leading to implant removal. While all TIVAPs appear to be colonized, only a fraction become infected, and the relationship between nonpathogenic organisms colonizing TIVAPs and the potential risk of infection is unknown. We explored bacteria present on TIVAPs implanted in patients with or without signs of TIVAP infection and identified differences in phylum composition and community structure. Our data suggest that the microbial ecology of intravascular devices could be predictive of TIVAP infection status and that ultimately a microbial ecological signature could be identified as a tool to predict TIVAP infection susceptibility and improve clinical management.
Databáze: OpenAIRE
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