Characterization of neutralizing versus binding antibodies and memory B cells in COVID-19 recovered individuals from India
| Autor: | Rafi Ahmed, Jens Wrammert, Carl W. Davis, Anmol Chandele, Sanjeev Kumar, Amit Sharma, Kaustuv Nayak, Robert C. Kauffman, Mehul S. Suthar, Kamalvishnu P. Gottimukkala, Susanne L. Linderman, Elluri Seetharami Reddy, Deepali Savargaonkar, Satyam Arora, Katharine Floyd, Grace Mantus, Kaja Murali-Krishna, Manju Rahi, Venkata Viswanadh Edara, Pawan Kumar Goel |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Adolescent Coronavirus disease 2019 (COVID-19) Vaccine evaluation Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) India Antibodies Viral Neutralizing antibodies Article Young Adult 03 medical and health sciences Immune system Virology Humans Neutralizing antibody Memory B cell Pandemics Aged 030304 developmental biology B-Lymphocytes 0303 health sciences biology SARS-CoV-2 030302 biochemistry & molecular biology COVID-19 Middle Aged Memory B cells Antibodies Neutralizing Immunity Humoral Immunoglobulin Isotypes Titer Spike Glycoprotein Coronavirus Immunology biology.protein Antibody Receptor binding domain |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2021.02.002 |
| Popis: | India is one of the most affected countries by COVID-19 pandemic; but little is understood regarding immune responses to SARS-CoV-2 in this region. Herein we examined SARS-CoV-2 neutralizing antibodies, IgG, IgM, IgA and memory B cells in COVID-19 recovered individual from India. While a vast majority of COVID-19 recovered individuals showed SARS-CoV-2 RBD-specific IgG, IgA and IgM antibodies (38/42, 90.47%; 21/42, 50%; 33/42, 78.57% respectively), only half of them had appreciable neutralizing antibody titers. RBD-specific IgG, but not IgA or IgM titers, correlated with neutralizing antibody titers and RBD-specific memory B cell frequencies. These findings have timely significance for identifying potential donors for plasma therapy using RBD-specific IgG assays as surrogate measurement for neutralizing antibodies in India. Further, this study provides useful information needed for designing large-scale studies towards understanding of inter-individual variation in immune memory to SARS CoV-2 natural infection for future vaccine evaluation and implementation efforts. |
| Databáze: | OpenAIRE |
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