Regulation of Hemangioblast Development
| Autor: | Gordon Keller, James Palis, Marion Kennedy, Scott M. Robertson, Georges Lacaud |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Vascular Endothelial Growth Factor A
Hematopoietic System Gestational Age Endothelial Growth Factors Biology Models Biological General Biochemistry Genetics and Molecular Biology Colony-Forming Units Assay Embryonic and Fetal Development Mice chemistry.chemical_compound History and Philosophy of Science Proto-Oncogene Proteins Basic Helix-Loop-Helix Transcription Factors Animals Humans Cell Lineage Cells Cultured T-Cell Acute Lymphocytic Leukemia Protein 1 Yolk Sac Mice Knockout Genetics Regulation of gene expression Lymphokines Vascular Endothelial Growth Factors Stem Cells General Neuroscience Gene Expression Regulation Developmental Cell Differentiation Hematopoietic Stem Cells Embryonic stem cell Hematopoiesis Cell biology DNA-Binding Proteins Endothelial stem cell Vascular endothelial growth factor A Haematopoiesis Liver RUNX1 chemistry Core Binding Factor Alpha 2 Subunit Hemangioblast Genes Lethal Endothelium Vascular Stem cell Transcription Factors |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1749-6632 0077-8923 |
| Popis: | The in vitro differentiation of embryonic stem (ES) cells provides a powerful approach for studying the earliest events involved in the commitment of the hematopoietic and endothelial lineages. Using this model system, we have identified a precursor with the potential to generate both primitive and definitive hematopoietic cells as well as cells with endothelial characteristics. The developmental potential of this precursor suggests that it represents the in vitro equivalent of the hemangioblast, a common stem cell for both lineages. ES cells deficient for the transcription factor scl/tal-1 are unable to generate hemangioblasts, while those deficient for Runx1 generate reduced numbers of these precursors. These findings indicate that both genes play pivotal roles at the earliest stages of hematopoietic and endothelial development. In addition, they highlight the strength of this model system in studying the function of genes in embryonic development. |
| Databáze: | OpenAIRE |
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