miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis
| Autor: | Kai-Lin Xing, Hong-Xin Zhou, Li-Li Lu, Xiao-Li Shi, Dong-Min Shi, Wei-Zhong Wu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Down-Regulation Mice SCID medicine.disease_cause Metastasis 03 medical and health sciences 0302 clinical medicine SALL4 Mice Inbred NOD Cell Line Tumor microRNA medicine Potency Animals Humans ERBB3 RNA Messenger Base Sequence Chemistry Liver Neoplasms DNA Helicases Nuclear Proteins Cell Biology medicine.disease Epithelial Cell Adhesion Molecule Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Neoplastic Stem Cells Thy-1 Antigens Stem cell Carcinogenesis Signal Transduction Transcription Factors |
| Zdroj: | Cellular signalling. 72 |
| ISSN: | 1873-3913 |
| Popis: | Epithelial-mesenchymal transition (EMT), a pivotal event during cancer progression such as relapse and metastasis, is positively correlated with the stemness potency of tumor cells. Our previous study showed that miR-296-5p attenuated EMT program of hepatocellular carcinoma cells (HCC) through NRG1/ERBB2/ERBB3 signaling. In the present study, we uncovered that miR-296-5p was able to inhibit the stemness potency of HCC by decreasing the number and size of tumorspheres, downregulating the expression of CSC biomarkers and hampering the ability of tumorigenesis in NOD/SCID mice. Brahma-related gene-1 (Brg1), as the target protein of miR-296-5p detected by bioinformatics methods, activates a series of downstream cascades through directly binding to Sall4 promoter and enhancing Sall4 transcription. Importantly, the higher expressions of Brg1 and Sall4 in tumor tissues of HCC patients suggest poorer prognoses after surgical extraction. In conclusion, miR-296-5p exerts an inhibitory effect on stemness potency of HCC cells via Brg1/Sall4 axis. |
| Databáze: | OpenAIRE |
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