Plasmodium falciparum: hrp3 promoter region is associated with stage-specificity and episomal recombination
| Autor: | Leah Turner, Carlos López-Estraño, Anusha M. Gopalakrishnan, Kasturi Haldar, Jean-Philippe Semblat, Dominique Mazier |
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| Rok vydání: | 2007 |
| Předmět: |
Green Fluorescent Proteins
Plasmodium falciparum Immunology Protozoan Proteins Transfection Article Apicomplexa Species Specificity Transcription (biology) parasitic diseases Gene expression Animals RNA Messenger Promoter Regions Genetic Recombination Genetic Genetics Messenger RNA Luminescent Agents biology Proteins RNA Promoter General Medicine biology.organism_classification Molecular biology Infectious Diseases Gene Expression Regulation Parasitology Recombination Plasmids |
| Zdroj: | Experimental Parasitology. 116:327-333 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.01.020 |
| Popis: | The asexual blood stage of Plasmodium falciparum in the human host is comprised of morphologically distinct ring, trophozoite and schizont stages, each of which possesses a distinct pattern of gene expression. Episomal promoter recombination has been recently reported in malaria parasites. We aim to investigate the nature of this process, and its relationship with promoter activity, by employing a series of nested deletions of the ring specific hrp3 promoter. Our results showed a discrete promoter region that is preferentially used for recombination. The P. falciparum hrp3 RNA gene is only seen in ring-stage parasites but deletion of the recombination region was associated with decreased ring-stage expression and concurrent detection of transcripts in trophozoite-stage parasites. Our results describe a ring-stage specific regulatory region possibly involved in episomal promoter recombination, suggesting that common sequences might mediate both processes. |
| Databáze: | OpenAIRE |
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