Nanosized zeolites as a perspective material for conductometric biosensors creation

Autor: I. S. Kucherenko, Nicole Jaffrezic-Renault, A. P. Soldatkin, Florence Lagarde, Sergei V. Dzyadevych, Berna Ozansoy Kasap, Burcu Akata Kurç, Oleksandr O. Soldatkin, Salih Kaan Kirdeciler
Přispěvatelé: Laboratory of Biomolecular Electrochemistry, National Academy of Sciences of Ukraine (NASU), Institute of High Technologies [Kyiv], Taras Shevchenko National University of Kyiv, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Cent Lab, Middle East Technical University [Ankara] (METU), Micro & Nanotechnol Dept, The authors gratefully acknowledge the financial support of this study by the Project European IRSES-NANODEV. National Academy of Sciences of Ukraine supported a part of this work in the frame of Scientific and Technical Government Program 'Sensor systems for medico-ecological and industrial-technological requirement: metrological support and experimental operation'.
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Materials science
ADSORPTION
Nanochemistry
Nanotechnology
02 engineering and technology
010402 general chemistry
Conductometric transducer
01 natural sciences
GLUCOSE
Adsorption
Materials Science(all)
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Monolayer
General Materials Science
Glucose oxidase
Ceramic
Enzyme adsorption
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Zeolite
biology
Nano Express
Mesoporous silica spheres
MONOLAYERS
[CHIM.MATE]Chemical Sciences/Material chemistry
Mesoporous silica
021001 nanoscience & nanotechnology
Condensed Matter Physics
Urease
0104 chemical sciences
Chemical engineering
Silicalite-1
IMMOBILIZATION
visual_art
visual_art.visual_art_medium
biology.protein
Acetylcholinesterase
ELECTROCHEMICAL BIOSENSORS
0210 nano-technology
Biosensor
Zdroj: Nanoscale Research Letters
Nanoscale Research Letters, SpringerOpen, 2015, 10, pp.Article Number: 209 ⟨10.1186/s11671-015-0911-6⟩
ISSN: 1931-7573
1556-276X
DOI: 10.1186/s11671-015-0911-6⟩
Popis: International audience; In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200 degrees C turned out to be the best one. Nanozeolites beta and L, zeolite L, MSS, and silicalite-1 (80 to 450 nm) were tested as the adsorbents for enzyme urease. The biosensors with all tested particles except zeolite L had good analytical characteristics. Silicalite-1 (450 nm) was also used for adsorption of glucose oxidase, acetylcholinesterase, and butyrylcholinesterase. The glucose and acetylcholine biosensors were successfully created, whereas butyrylcholinesterase was not adsorbed on silicalite-1. The enzyme adsorption on zeolites and MSS is simple, quick, well reproducible, does not require use of toxic compounds, and therefore can be recommended for the development of biosensors when these advantages are especially important.
Databáze: OpenAIRE
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