Piperlongumine inhibits TGF-β-induced epithelial-to-mesenchymal transition by modulating the expression of E-cadherin, Snail1, and Twist1
| Autor: | Jong Kil Lee, Min Ju Park, Jong Ho Kim, Seo Young Jeong, Man Kyu Shim, Da Eun Lee, Eun Hyang Jang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Down-Regulation Motility Biology Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Transforming Growth Factor beta medicine Humans Neoplasm Invasiveness Epithelial–mesenchymal transition Piperlongumine Cell Proliferation Pharmacology Cadherin Twist-Related Protein 1 Mesenchymal stem cell Cancer Dioxolanes Cadherins medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology chemistry 030220 oncology & carcinogenesis Cancer cell MCF-7 Cells Cancer research Snail Family Transcription Factors |
| Zdroj: | European Journal of Pharmacology. 812:243-249 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2017.07.036 |
| Popis: | Cancer is a life-threatening disease, and the occurrence of metastasis, which increases the lethality of primary tumors, is increasing. The epithelial-to-mesenchymal transition (EMT) is a biological process by which epithelial cells lose cell-cell adhesion properties and acquire mesenchymal properties, including motility and invasiveness. EMT is considered an early stage of metastasis; therefore, inhibiting EMT may be an effective anticancer therapy. In the present study, the antimetastatic effect of piperlongumine (PL) was assessed in human cancer cells. PL is a single component isolated from long pepper (Piper longum) and it has been studied for its antibacterial, antiangiogenic, and antidiabetic activities. Migration assays (wound healing assay) and transwell invasion assays showed that PL inhibited the migration and invasion of cancer cells. Western blotting and immunofluorescence imaging showed that TGF-β upregulated the transcription factors Snail1 and Twist1 and downregulated E-cadherin, a marker of epithelial cells, inducing EMT. PL might inhibit TGF-β-induced EMT by downregulating Snail1 and Twist1 and upregulating E-cadherin in cancer cells. In summary, PL might inhibit TGF-β-induced EMT, suggesting that it is a promising anticancer agent. |
| Databáze: | OpenAIRE |
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