DNA aptamer-based sorbents for binding human IgE

Autor:	Olga I. Afanasieva, E. D. Ovchinnikova, K. A. Glinkina, P. A. Levashov, V. A. Spiridonova, S.N. Pokrovsky, I. Yu. Adamova
Rok vydání:	2014
Předmět:	Chromatography Sorbent biology Aptamer Organic Chemistry Oligonucleotides DNA Aptamers Nucleotide Immunoglobulin E Biochemistry Matrix (chemical analysis) chemistry.chemical_compound chemistry Human ige Desorption biology.protein Humans Bioorganic chemistry Protein Binding
Zdroj:	Russian Journal of Bioorganic Chemistry. 40:151-154
ISSN:	1608-330X 1068-1620
DOI:	10.1134/s1068162014020125
Popis:	DNA aptamer-based sorbents were synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were within the range of 1.1 × 10−10 to 1.7 × 10−10 M depending on the orientation of the aptamer on an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were selected. These chromatographic materials can be used for medical and biotechnological applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecf68938748633f1c7960a157372773e https://doi.org/10.1134/s1068162014020125 Zobrazit plný text záznamu Full text from SpringerLink