A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia

Autor: Wynter, C., Patel, B.K.C., Bain, Peter, De Jersey, J., Hamilton, S., Inkerman, P.A.
Rok vydání: 1996
Předmět:
Zdroj: FEMS Microbiology Letters
ISSN: 1574-6968
0378-1097
DOI: 10.1111/j.1574-6968.1996.tb08348.x
Popis: A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 °C and a pH optimum between 5 and 6. The half life was ~ 6.5 h at 75 °C and 2 h at 80 °C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB1 lAd was a member of the genus Thermoanaerobacter.
Databáze: OpenAIRE
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