Exosome-loaded extracellular matrix-mimic hydrogel with anti-inflammatory property Facilitates/promotes growth plate injury repair
| Autor: | Zheng Chen, Yongchang Lin, Can Liu, Lei Fan, Qiyou Wang, Pengfei Guan, Denghui Xie, Yongjian Sun, Yuelun Ji, Shichao Mao |
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| Rok vydání: | 2022 |
| Předmět: |
Immunomodulatory
food.ingredient Anabolism QH301-705.5 Biomedical Engineering Inflammation Exosomes Gelatin Exosome Article Chondrocyte Biomaterials Extracellular matrix chemistry.chemical_compound food medicine Chondroitin sulfate Biology (General) Materials of engineering and construction. Mechanics of materials Chemistry Regeneration (biology) Extracellular matrix-mimic hydrogel Cell biology medicine.anatomical_structure TA401-492 Growth plate injury repair medicine.symptom Extracellular matrix deposition Biotechnology |
| Zdroj: | Bioactive Materials Bioactive Materials, Vol 10, Iss, Pp 145-158 (2022) |
| ISSN: | 2452-199X |
| DOI: | 10.1016/j.bioactmat.2021.09.010 |
| Popis: | Growth plate cartilage has limited self-repair ability, leading to poor bone bridge formation post-injury and ultimately limb growth defects in children. The current corrective surgeries are highly invasive, and outcomes can be unpredictable. Following growth plate injury, the direct loss of extracellular matrix (ECM) coupled with further ECM depletion due to the inhibitory effects of inflammation on the cartilage matrix protein greatly hinder chondrocyte regeneration. We designed an exosome (Exo) derived from bone marrow mesenchymal stem cells (BMSCs) loaded ECM-mimic hydrogel to promote cartilage repair by directly supplementing ECM and anti-inflammatory properties. Aldehyde-functionalized chondroitin sulfate (OCS) was introduced into gelatin methacryloyl (GM) to form GMOCS hydrogel. Our results uncovered that GMOCS hydrogel could significantly promote the synthesis of ECM due to the doping of OCS. In addition, the GMOCS-Exos hydrogel could further promote the anabolism of chondrocytes by inhibiting inflammation and ultimately promote growth plate injury repair through ECM remodeling. Graphical abstract Image 1 Highlights • Chondrocytes are difficult to regenerate after growth plate injury due to extensive degradation of ECM (extracellular matrix). • GMOCS-Exos can promote the synthesis of ECM by directly supplementing ECM and anti-inflammatory properties. • GMOCS-Exos can boost cartilage regeneration after growth plate injury and reduce bone bridge formation. |
| Databáze: | OpenAIRE |
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