Massively multiplex chemical transcriptomics at single-cell resolution

Autor:	Lena Christiansen, Lauren M. Saunders, Vijay Ramani, Dana Jackson, Hannah A. Pliner, Jay Shendure, Cole Trapnell, Frank J. Steemers, Jonathan S. Packer, José L. McFaline-Figueroa, Fan Zhang, Riza M. Daza, Sanjay Srivatsan, Junyue Cao
Rok vydání:	2020
Předmět:	Resolution (mass spectrometry) Cell Computational biology Acetates Biology Article Transcriptome Neoplasms medicine Humans Multiplex RNA-Seq Cell Nucleus Multidisciplinary Genomics Chromatin High-Throughput Screening Assays Histone Deacetylase Inhibitors medicine.anatomical_structure A549 Cells Cancer cell MCF-7 Cells Histone deacetylase Single-Cell Analysis K562 Cells Intracellular
Zdroj:	Science
ISSN:	1095-9203 0036-8075
Popis:	Single-cell chemical transcriptomics Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular heterogeneity at the resolution of individual cells. Srivatsan et al. now add another layer of information and complexity by combining single-cell transcriptomics with oligo hashing and small molecule screening in a method called sci-Plex. Because screening many chemical compounds requires the ability to profile many cells, and because screens perturb cells in many different ways, the authors demonstrate the effects of 188 compounds in three cancer lines. The sci-Plex method can capture gene expression profiles from thousands of experimental conditions in a single experiment. Science , this issue p. 45
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecf2683662eb6b8785c1a44f5749bf05 https://doi.org/10.1126/science.aax6234 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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