Genetic variability of human metapneumo- and bocaviruses in children with respiratory tract infections

Autor: Afroditi Moutousi, Athanasios Kossyvakis, Vasiliki Pogka, Dionyssios N. Sgouras, Maria Giannaki, Antonios Kalliaropoulos, Andreas Mentis
Přispěvatelé: Laboratoire national de référence pour la grippe dans le sud de la Grèce = National Influenza Reference Laboratory of Southern Greece [Athens] (NIRLSG), Institut Pasteur Hellénique, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Laboratoire de microbiologie médicale = Laboratory of Medical Microbiology [Athènes], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Aghia Sophia Children's Hospital, National and Kapodistrian University of Athens (NKUA)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
MESH: Sequence Analysis
DNA
Epidemiology
viruses
Sequence Homology
MESH: Parvoviridae Infections/virology
MESH: Viral Structural Proteins/genetics
Genetic analysis
MESH: Genotype
Human bocavirus
MESH: Child
MESH: Parvoviridae Infections/epidemiology
Genotype
Cluster Analysis
MESH: DNA
Viral/genetics
MESH: Sequence Homology
Child
MESH: Phylogeny
Respiratory Tract Infections
Phylogeny
Genetics
Molecular Epidemiology
MESH: Human bocavirus/genetics
Paramyxoviridae Infections
Respiratory tract infections
Phylogenetic tree
Greece
MESH: Genetic Variation
MESH: DNA
Viral/chemistry
MESH: Infant
Newborn
virus diseases
MESH: Respiratory Tract Infections/epidemiology
MESH: Infant
3. Good health
Infectious Diseases
MESH: Paramyxoviridae Infections/virology
Child
Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA
Viral
Female
MESH: RNA
Viral/genetics
MESH: Greece/epidemiology
Pulmonary and Respiratory Medicine
Adolescent
MESH: Human bocavirus/classification
Molecular Sequence Data
Biology
MESH: Metapneumovirus/isolation & purification
Parvoviridae Infections
Bocavirus
children
MESH: Metapneumovirus/genetics
MESH: Paramyxoviridae Infections/epidemiology
respiratory viruses
Genetic variation
Humans
MESH: Molecular Epidemiology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Genetic variability
MESH: Human bocavirus/isolation & purification
Viral Structural Proteins
MESH: Adolescent
MESH: Metapneumovirus/classification
MESH: Molecular Sequence Data
MESH: Humans
Molecular epidemiology
phylogenetic analysis
MESH: Child
Preschool
Public Health
Environmental and Occupational Health
Infant
Newborn
Genetic Variation
Infant
Original Articles
Sequence Analysis
DNA
biology.organism_classification
metapneumovirus
Virology
MESH: Cluster Analysis
MESH: Male
DNA
Viral
MESH: Female
MESH: Respiratory Tract Infections/virology
Zdroj: Influenza and Other Respiratory Viruses
Influenza and Other Respiratory Viruses, Wiley Open Access, 2014, 8 (1), pp.107-115. ⟨10.1111/irv.12185⟩
ISSN: 1750-2640
1750-2659
DOI: 10.1111/irv.12185⟩
Popis: Objectives The genotypic analysis of human metapneumo-(HMPV) and boca-(HBoV) viruses circulating in Greece and their comparison to reference and other clinical strains. Design Genetic analysis of representative strains over three consecutive winter seasons of the years 2005–2008. Setting Representative positive specimens for HMPV and HBoV from paediatric patients of healthcare units and hospitals in Southern Greece with influenza-like illness or other respiratory tract infections. Sample Seven to ten positive specimens for either HMPV or HBoV from each winter period. In total, 24 specimens positive for HMPV and 26 for HBoV, respectively. Main outcome measures Sequence diversity of HMPV and HBoV strains by sequencing the complete G and VP1/VP2 genes, respectively. Results In total, 24 HMPV strains were found to have a 92–100% nucleotide and a 85.9–100% amino acid identity. Phylogenetic analysis based on the number of amino acid differences, revealed circulation of 4 different subclusters belonging to genetic lineage B2. Similarly, analysis of 26 HBoV strains indicated that 22 clustered within genotype St2, 2 into genotype St1 and the remaining 2 formed a third cluster derived from potential recombination between different St1 genotype strains. St2 HBoV genotype was observed throughout the whole observation period whereas St1 only during the second and the third winter period. Higher levels of heterogeneity were observed between HMPV compared to HBoV strains. Conclusions Phylogenetic analysis revealed circulation of one single lineage (B2) for HMPV viruses and predominance of St2 genotype for HBoV viruses. A possible recombination between St1 genotype strains of HBoV was observed.
Databáze: OpenAIRE
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