Inhibition of eosinophil activation in bronchoalveolar lavage fluid from atopic asthmatics by Y-24180, an antagonist to platelet-activating factor
| Autor: | Shuichi Iwane, Youichi Akiyama, Masami Mizuki, Hirotsugu Komatsu, Tomiyasu Tsuda |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Integrin alpha1 Macrophage-1 Antigen Bronchi General Biochemistry Genetics and Molecular Biology Leukocyte Count chemistry.chemical_compound Ribonucleases Antigens CD Eosinophil activation medicine Humans Platelet Activating Factor General Pharmacology Toxicology and Pharmaceutics Eosinophil cationic protein medicine.diagnostic_test Platelet-activating factor biology Cell adhesion molecule Chemistry Azepines Blood Proteins General Medicine Eosinophil Granule Proteins Middle Aged Triazoles respiratory system Eosinophil Intercellular Adhesion Molecule-1 Asthma Fibronectins Eosinophils Bronchoalveolar lavage medicine.anatomical_structure Integrin alpha M Immunology biology.protein Female Bronchoalveolar Lavage Fluid Alpha chain |
| Zdroj: | Life Sciences. 65:2031-2039 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(99)00470-1 |
| Popis: | We examined the effects of Y-24180, a potent and long-acting antagonist to platelet-activating factor (PAF) receptor, on the expression of adhesion molecules in peripheral blood and bronchoalveolar lavage fluid (BALF) eosinophils from atopic asthmatics. Y-24180 (20 mg/day) was administered to 4 atopic asthmatics for 8 weeks. The number of eosinophils, the level of eosinophil cationic protein (ECP), the bindings of soluble intercellular adhesion molecule-1 (sICAM-1) and fibronectin (FN), and the expressions of CD11b (alpha chain of Mac-1) and CD49d (alpha chain of VLA-4) on eosinophils were evaluated in peripheral blood (n=4) and BALF (n=3) before and after the administration of Y-24180. The infiltration of eosinophils into the bronchial wall was also examined by taking biopsies. Eosinophil count, sICAM-1 and FN binding to eosinophils in BALF significantly decreased after the administration of Y-24180 (p0.05). The level of CD11b expression also decreased remarkably after the administration (n=2). In peripheral blood, eosinophil count and ECP level did not change. The binding of sICAM-1 and FN, and expression of CD11b on eosinophils in peripheral blood showed a tendency to decrease after the administration. The level of CD49d expression on eosinophils changed neither in BALF nor in blood. Eosinophil infiltration into the bronchial wall markedly decreased in one out of 3 cases after the administration. These results suggest that Y-24180 inhibits the activation of eosinophils by antagonizing the actions of PAF in atopic asthmatics. |
| Databáze: | OpenAIRE |
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