Effect of Lactobacillus rhamnosus GG Supplementation on Intestinal Inflammation Assessed by PET/MRI Scans and Gut Microbiota Composition in HIV-Infected Individuals

Autor: Johannes R. Hov, Eva Fallentin, Susanne Dam Nielsen, Sofie Jespersen, Andreas Kjaer, Caroline J Arnbjerg, Kristian Holm, Bente Halvorsen, Barbara M. Fischer, Beate Vestad, Karin K. Pedersen, Theis Lange, Adam E. Hansen, Helle Hjorth Johannesen, Marius Trøseid
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
DNA
Bacterial
Male
Inflammation
HIV Infections
Gut flora
digestive system
DNA
Ribosomal
law.invention
03 medical and health sciences
Probiotic
fluids and secretions
Lactobacillus rhamnosus
Intestinal inflammation
law
Hiv infected
RNA
Ribosomal
16S
medicine
Animals
Humans
Pharmacology (medical)
Prospective Studies
Phylogeny
biology
business.industry
Lacticaseibacillus rhamnosus
Probiotics
digestive
oral
and skin physiology
Gastrointestinal Microbiome
food and beverages
Sequence Analysis
DNA
Middle Aged
biology.organism_classification
Magnetic Resonance Imaging
Gut microbiome
Enteritis
030104 developmental biology
Infectious Diseases
Treatment Outcome
Bacterial Translocation
Positron-Emission Tomography
Immunology
Dysbiosis
Female
medicine.symptom
business
Biomarkers
Zdroj: Journal of acquired immune deficiency syndromes (1999). 78(4)
ISSN: 1944-7884
Popis: Alterations in the gut microbiome have been associated with inflammation and increased cardiovascular risk in HIV-infected individuals. The aim of this study was to investigate the effects of the probiotic strain Lactobacillus rhamnosus GG (LGG) on intestinal inflammation, gut microbiota composition, and systemic markers of microbial translocation and inflammation in HIV-infected individuals.This prospective, clinical interventional trial included 45 individuals [15 combination antiretroviral treatment (cART) naive and 30 cART treated] who ingested LGG twice daily at a dosage of 6 × 109 colony-forming units per capsule for a period of 8 weeks. Intestinal inflammation was assessed using F-2-fluoro-2-deoxy-D-glucose positron emission tomography/magnetic resonance imaging (F-FDG PET/MRI) scans in 15 individuals. Gut microbiota composition (V3-V4 region of the 16s rRNA gene) and markers of microbial translocation and inflammation (lipopolysaccharide, sCD14, sCD163, sCD25, high-sensitive CRP, IL-6, and tumor necrosis factor-alpha) were analyzed at baseline and after intervention.At baseline, evidence of intestinal inflammation was found in 75% of the participants, with no significant differences between cART-naive and cART-treated individuals. After LGG supplementation, a decrease in intestinal inflammation was detected on PET/MRI (-0.3 mean difference in the combined activity grade score from 6 regions, P = 0.006), along with a reduction of Enterobacteriaceae (P = 0.018) and Erysipelotrichaceae (P = 0.037) in the gut microbiome, with reduced Enterobacteriaceae among individuals with decreased F-FDG uptake on PET/MRI (P = 0.048). No changes were observed for soluble markers of microbial translocation and inflammation.A decrease in intestinal inflammation was found in HIV-infected individuals after ingestion of LGG along with a reduced abundance of Enterobacteriaceae, which may explain the local anti-inflammatory effect in the gut.
Databáze: OpenAIRE
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