Systemic treatment with 4-At-211-phenylalanine enhances survival of rats with intracranial glioblastoma
| Autor: | J. K. Krauss, Almut Walte, N. Borrmann, Makoto Nakamura, H. J. Hedrich, S. Friedrich, Wolfram H. Knapp, G.-J. Meyer, K. Schwabe |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Proliferation index Phenylalanine Brachytherapy Tumor resection Urology 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Animals Radiology Nuclear Medicine and imaging Microinjection Brain Neoplasms business.industry Health condition Dose-Response Relationship Radiation General Medicine medicine.disease Rats 3. Good health Survival Rate Treatment Outcome 030220 oncology & carcinogenesis Immunohistochemistry Female Radiopharmaceuticals medicine.symptom Glioblastoma business Astatine 030217 neurology & neurosurgery Increased amino acid |
| Zdroj: | Nuklearmedizin; Vol 52 |
| ISSN: | 0029-5566 |
| DOI: | 10.3413/Nukmed-0580-13-05 |
| Popis: | SummaryObjective: Increased amino acid transport in brain tumours is used for diagnostic purposes. It has been shown that the α-emitting radionuclide astatine-211 labeled to L-phenylalanine is taken up by glioblastoma cells. We here tested, if systemic treatment with 4-[211At] astatine-phenylalanine (At-Phe) has a beneficial effect on survival of rats with intracranial glioblastoma. Animals, methods: The rat glioblastoma cell line BT4Ca was implanted into the prefrontal cortex of female BDIX rats by stereotaxic microinjection (10 000 cells/3 μl; n = 83). 3 days after implantation At-Phe or phosphate buffered saline were injected intravenously. A third group was treated twice, i.e., on day 3 and 10. Health condition was assessed each day by using a score system. Rats were sacrificed on days 6, 10, 13 and 17 after implantation, or when showing premortal health condition to measure tumour volume and necrosis. The proliferation index (PI) was assessed after immunohistochemical staining of Ki-67. Results: Survival time of rats treated twice with At-Phe was significantly prolonged. Additionally, both At-Phe-treated groups remained significantly longer in a better health condition. Rats with poor health status had larger tumours than rats with fair health condition. Overall, irrespective of treatment the PI was reduced in rats with poor health condition. Necrosis was larger in rats treated twice with At-Phe. Conclusion: Intravenous treatment with At-Phe enhanced survival time of rats with intracranial glioblastomas and improved health condition. These results encourage studies using local treatment of intracranial glioblastoma with At-Phe, either by repeated local injection or by intracavital application after tumour resection. |
| Databáze: | OpenAIRE |
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