Molecular regulation of transferrin receptor and ferritin expression in the rat gastrointestinal tract
| Autor: | Tracey L. Allen, Kerrie A Basclain, Gary P. Jeffrey |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Colon Iron Crypt Molecular Sequence Data Transferrin receptor In situ hybridization Polymerase Chain Reaction Epithelium Rats Sprague-Dawley Intestine Small Receptors Transferrin medicine Animals Large intestine RNA Messenger Receptor In Situ Hybridization chemistry.chemical_classification Hepatology biology Base Sequence Stomach Gastroenterology Iron Deficiencies Molecular biology Immunohistochemistry Small intestine Rats Up-Regulation Ferritin medicine.anatomical_structure Biochemistry chemistry Transferrin Gastric Mucosa Ferritins biology.protein Digestive System |
| Zdroj: | Gastroenterology. 110(3) |
| ISSN: | 0016-5085 |
| Popis: | BACKGROUND & AIMS: The role of the transferrin receptor and ferritin i n the regulation of intestinal dietary iron uptake and excretion is unknown. This study aimed to determine the regulation of transferrin receptor and ferritin messenger RNA (mRNA) in the rat gastrointestinal tract in response to dietary iron changes. METHODS: In situ hybridization studies for transferrin receptor and L-ferritin mRNAs were performed using tissues from normal-iron-deficient, and iron- loaded rats. RESULTS: L-ferritin mRNA was localized to small intestinal crypt and villus epithelial cells and colonic crypt and surface epithelial cells with mRNA levels up-regulated in iron-loaded rats. Transferrin receptor mRNA was detected in crypt epithelial cells of the small and large intestine in iron-deficient and normal rats. In contrast, in iron-loaded rats, transferrin receptor mRNA was also detected in the superficial epithelial cells of the small intestine and colon, which contained increased stores of iron. Transferrin receptor mRNA levels were increased in the colon. CONCLUSIONS: In the iron- deficient and normal rat intestine, transferrin receptor mRNA was expressed only by proliferating crypt epithelial cells. In iron-loaded rats, however, surface enterocytes of the intestine expressed both transferrin receptor mRNA and increased ferritin mRNA levels. (Gastroenterology 1996 Mar;110(3):790-800) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|