Drug preconcentration and direct quantification in biofluids using 3D-Printed paper cartridge
| Autor: | Jie Tian, Guoqian Li, Langlang Yi, Gang Zhao, Lei Zhao, Jianduo Wu, Bo Hu, Zhixiang Xiao, Cheng He |
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| Rok vydání: | 2020 |
| Předmět: |
Drug
3d printed Materials science Silver media_common.quotation_subject Biomedical Engineering Biophysics 02 engineering and technology Biosensing Techniques Silver nanowires Spectrum Analysis Raman 01 natural sciences Drug detection Cartridge Electrochemistry Animals media_common Chromatography 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Anticancer drug 0104 chemical sciences Artificial urine Pharmaceutical Preparations Clinical diagnosis Printing Three-Dimensional Cattle 0210 nano-technology Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 189 |
| ISSN: | 1873-4235 |
| Popis: | Drug detection in biofluids has always been great importance for clinical diagnosis. Many detection technologies such as chromatography-mass spectrometry, have been applied to the detection of drugs. However, these technologies required multi-step operations, including complicated and time-consuming pretreatment processes and operations of bulky detection instruments, significantly limiting development of drug detection in clinical diagnosis. Herein, a portable 3D-printed paper cartridge was fabricated for fast sample preconcentration and direct drugs quantitative detection in biofluids by a portable Raman spectrometer. This cartridge contained both paper tip with silver nanowires to preconcentrate samples and achieve surface-enhanced Raman Scattering (SERS) measurement, and 3D-printed cartridge to build enclosed environment for the improvement of detection, which cost only one dollar. The preconcentration ability of the cartridge was up to 18.13-fold fluorescence enhancement and compared to the non-preconcentration method, it achieved 9.93-fold improvement of SERS performance. The anticancer drug of epirubicin hydrochloride, cyclophosphamide and their mixtures were quantitatively detected in the bovine serum or artificial urine. The integrated detection procedure required only 1 h, including sample pretreatment and preconcentration, drying, SERS measurements, and quantification analysis. This 3D-printed paper cartridge constituted a portable detection platform that would be potentially a practical and point-of-care detection tool for drug preconcentration and quantification on the clinical diagnosis. |
| Databáze: | OpenAIRE |
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