Reprogramming the phenylpropanoid metabolism in seeds of oilseed rape by suppressing the orthologs of reduced epidermal fluorescence1
| Autor: | Juliane Mittasch, Andrej Frolov, Christoph Böttcher, Carsten Milkowski, Dieter Strack |
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| Rok vydání: | 2013 |
| Předmět: |
Sinapaldehyde
Physiology Propanols education Molecular Sequence Data Plant Science Genes Plant behavioral disciplines and activities complex mixtures Choline chemistry.chemical_compound Biosynthesis Biochemistry and Metabolism Arabidopsis Sinapine Genetics Arabidopsis thaliana Chromatography High Pressure Liquid Crosses Genetic Plant Proteins biology Phenylpropanoid Sequence Homology Amino Acid Brassica napus Homozygote food and beverages Brassicaceae Esters social sciences Aldehyde Dehydrogenase biology.organism_classification Plants Genetically Modified Biosynthetic Pathways Blotting Southern chemistry Biochemistry Seeds Metabolome Kaempferol Genome Plant |
| Zdroj: | Plant physiology. 161(4) |
| ISSN: | 1532-2548 |
| Popis: | As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene REDUCED EPIDERMAL FLUORESCENCE1 (REF1) encoding a coniferaldehyde/sinapaldehyde dehydrogenase. The enzyme is involved in the formation of ferulate and sinapate from the corresponding aldehydes, thereby linking lignin and hydroxycinnamate biosynthesis as a potential branch-point enzyme. We used RNA interference to silence REF1 genes in seeds of oilseed rape. Nontargeted metabolite profiling showed that BnREF1-suppressing seeds produced a novel chemotype characterized by reduced levels of sinapate esters, the appearance of conjugated monolignols, dilignols, and trilignols, altered accumulation patterns of kaempferol glycosides, and changes in minor conjugates of caffeate, ferulate, and 5-hydroxyferulate. BnREF1 suppression affected the level of minor sinapate conjugates more severely than that of the major component sinapine. Mapping of the changed metabolites onto the phenylpropanoid metabolic network revealed partial redirection of metabolic sequences as a major impact of BnREF1 suppression. |
| Databáze: | OpenAIRE |
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