Effect of amphotericin B and voriconazole on the outgrowth of conidia of Aspergillus fumigatus followed by time-lapse microscopy
Autor: | Laszlo Talas, Gaspar Banfalvi, Gábor Szemán-Nagy, Zsuzsa M. Szigeti |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
Hyphal growth Attachment of conidia Hypha lcsh:Biotechnology Biophysics lcsh:QR1-502 Spatio-temporal analysis 01 natural sciences Applied Microbiology and Biotechnology lcsh:Microbiology Microbiology Aspergillus fumigatus Conidium 03 medical and health sciences Brown’s movement Image processing 010608 biotechnology Amphotericin B lcsh:TP248.13-248.65 medicine Candida albicans 030304 developmental biology Voriconazole 0303 health sciences biology fungi biology.organism_classification Spore Original Article medicine.drug |
Zdroj: | AMB Express, Vol 9, Iss 1, Pp 1-8 (2019) AMB Express |
ISSN: | 2191-0855 |
Popis: | Studies of morphological measurements from the outgrowth of cells to a network of hyphae have been extended from Candida albicans (Nagy et al. in Appl Microbiol Biotechnol 98(11):5185–5194. https://doi.org/10.1007/s00253-014-5696-5 , 2014) to invasive conidiospores of Aspergillus fumigatus upon treatment with antifungal agents. The understanding of mycelial processes is important to optimize industrial processes such as fermentation and contributes to the fight against pathogenic fungi. This brief study combines TLS with digital image analysis. The TLS system was adapted to get information related to the adherence and growth dynamics of filamentous fungi. This approach was used earlier to distinguish among subphases of bacterial and fungal infections of mammal cells by detecting Mycoplasma infection in cell cultures causing serious damages in cell cultures. We describe changes in adherence, germination of spores, and hyphal growth of A. fumigatus, taking place in the absence and presence of amphotericin B (AMB) and voriconazole (VRC). These growth parameters were measured by TLS in CO2 incubator under physiological Photomicrography by TLS and extended for a longer period of time up to several weeks combined with image analysis represents a comfortable and reliable means to characterize the growth dynamism of A. fumigatus. The most important observation of medical importance related to the pathomechanism of VRC was that it did not adhere to conidiospores, i.e. that it did not contribute to the attachment of spores to the growth surface, and did not prevent germination but delayed hypha protrusion and elongation. In contrast AMB adhered to conidia, inhibited germination, hypha elongation and branching. It was concluded that AMB was efficient against the therapy of growth but not against the prevention of fungal infection. |
Databáze: | OpenAIRE |
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