A Drosophila natural variation screen identifies NKCC1 as a substrate of NGLY1 deglycosylation and a modifier of NGLY1 deficiency
| Autor: | Aylin R. Rodan, Raghavendran Partha, Katie G Owings, Emily Coelho, Nathan L. Clark, Cathleen M. Lutz, Aamir Zuberi, Clement Y. Chow, Gaelle Mercenne, Dana M Talsness, John M. Pleinis |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Genetics
chemistry.chemical_classification 0303 health sciences Substrate (chemistry) Biology Phenotype 03 medical and health sciences 0302 clinical medicine Enzyme chemistry Cytoplasm 030220 oncology & carcinogenesis NGLY1 Ion transporter Function (biology) 030304 developmental biology Genetic association |
| DOI: | 10.1101/2020.04.13.039651 |
| Popis: | N-Glycanase 1 (NGLY1) is a cytoplasmic deglycosylating enzyme. Loss-of-function mutations in theNGLY1gene cause NGLY1 deficiency, which is characterized by developmental delay, seizures, and a lack of sweat and tears. To model the phenotypic variability observed among patients, we crossed aDrosophilamodel of NGLY1 deficiency onto a panel of genetically diverse strains. The resulting progeny showed a phenotypic spectrum from 0-100% lethality. Association analysis on the lethality phenotype as well as an evolutionary rate covariation analysis generated lists of modifying genes, providing insight into NGLY1 function and disease. The top association hit wasNcc69(humanNKCC1/2), a conserved ion transporter. Analyses in NGLY1 -/- mouse cells demonstrated that NKCC1 is misglycosylated and has reduced function, making it only the second confirmed NGLY1 enzymatic substrate. The misregulation of this ion transporter may explain the observed defects in secretory epithelium function in NGLY1 deficiency patients. |
| Databáze: | OpenAIRE |
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