Karyomorphology And Nuclear Dna Content Of Sixteen Ophrys L. Taxa From Turkey
| Autor: | Ekrem Sezik, İsmail Gökhan Deniz, İlker Genç, Gülru Yücel, Hüseyin Sümbül, Metin Tuna |
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| Přispěvatelé: | Deniz, İ.G., Genç, İ., Yücel, G., Sümbül, H., Sezik, E., Tuna, M., Yeditepe Üniversitesi |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Orchidaceae biology Secondary constriction Karyotype Plant Science biology.organism_classification 010603 evolutionary biology 01 natural sciences Chromosome number secondary constriction Nuclear DNA 03 medical and health sciences 030104 developmental biology Taxon Genus DNA content Botany karyotype asymmetry Metaphase Ecology Evolution Behavior and Systematics Ophrys |
| Popis: | Karyotypes in 16 representative taxa of the Ophrys genus are compared, based on Feulgen-stained somatic metaphase chromosomes. The karyotypes of O. omegaifera subsp. israelitica, O. ulupinara, O. lycia, O. argolica subsp. lucis, O. argolica subsp. lesbis, O. climacis and O. reinholdii subsp. reinholdii are described for the first time. Karyological analyses indicate relationships among the species with respect to their asymmetry indices. All Ophrys taxa studied were diploid with 2n = 2x = 36 chromosomes. One B chromosome has been detected among the chromosomes of O. argolica subsp. lucis. All karyotypes are symmetrical, consisting of metacentric and submetacentric chromosomes. The longest chromosomes of all the investigated specimens contain a secondary constriction. It is determined that there is a correlation between the total number of chromosomes having secondary constrictions and the evolutionary development order of the taxa. Based on nuclear DNA content, analysis was carried out by flow cytometer using propodium iodide as fluorochrome, 2C nuclear DNA content of 16 Ophrys species varying between 20.80 pg (O. argolica subsp. lucis) and 23.11 pg (O. omegaifera subsp. israelitica). Karyotype asymmetry relationships are discussed according to the bidimensional scatter plots of A1–A2, CVCL–CVCI, CVCL–MCA and CVCI–MCA. © 2017 Società Botanica Italiana. |
| Databáze: | OpenAIRE |
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