Lithium inhibits a late step in agrin-induced AChR aggregation
| Autor: | B. G. Wallace, Shiv K. Sharma |
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| Rok vydání: | 2002 |
| Předmět: |
Indoles
Time Factors animal structures Blotting Western Muscle Fibers Skeletal Nerve Tissue Proteins Chick Embryo Lithium Biology Fluorescence Neuromuscular junction Cellular and Molecular Neuroscience chemistry.chemical_compound Culture Techniques medicine Animals Drug Interactions Receptors Cholinergic Agrin Enzyme Inhibitors Phosphorylation beta Catenin Acetylcholine receptor Dose-Response Relationship Drug Myogenesis General Neuroscience Receptor Aggregation Autophosphorylation Receptor Protein-Tyrosine Kinases Tyrosine phosphorylation Benzazepines Protein-Tyrosine Kinases Bungarotoxins musculoskeletal system Cell biology Cytoskeletal Proteins medicine.anatomical_structure nervous system Biochemistry chemistry Trans-Activators Signal transduction |
| Zdroj: | Journal of Neurobiology. 54:346-357 |
| ISSN: | 1097-4695 0022-3034 |
| Popis: | Agrin activates an intracellular signaling pathway to induce the formation of postsynaptic specializations on muscle fibers. In myotubes in culture, this pathway has been shown to include autophosphorylation of the muscle-specific kinase MuSK, activation of Src-family kinases, tyrosine phosphorylation of the acetylcholine receptor (AChR) β subunit, a decrease in receptor detergent extractability, and the accumulation of AChRs into high-density aggregates. Here we report that treating chick myotubes with lithium prevented any detectable agrin-induced change in AChR distribution without affecting the number of AChRs or the agrin-induced change in AChR tyrosine phosphorylation and detergent extractability. Lithium treatment also increased the rate at which AChR aggregates disappeared when agrin was removed. The effects of lithium developed slowly over the course of approximately 12 h. Thus, sensitivity to lithium identifies a late step in the agrin signaling pathway, after agrin-induced MuSK and AChR phosphorylation, that is necessary for the recruitment of AChRs into visible aggregates. © 2002 Wiley Periodicals, Inc. J Neurobiol 54: 346–357, 2003 |
| Databáze: | OpenAIRE |
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