The effect of hyaluronan on CD44-mediated survival of normal and hydroxyl radical-damaged chondrocytes
| Autor: | Roberta Cortivo, S Panfilo, Paola Brun, D. Daga Gordini, Giovanni Abatangelo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cartilage
Articular Xanthine Oxidase Cell Survival Neutrophils Biomedical Engineering Chondrocyte chemistry.chemical_compound Chondrocytes Rheumatology medicine Humans Orthopedics and Sports Medicine Viability assay CD44 Hyaluronic Acid Xanthine oxidase Receptor Hypoxanthine Hyaluronan Cells Cultured biology Dose-Response Relationship Drug Chemistry Histology Molecular biology Immunohistochemistry Microscopy Electron medicine.anatomical_structure Hyaluronan Receptors Biochemistry biology.protein Antibody |
| Zdroj: | Osteoarthritis and cartilage. 11(3) |
| ISSN: | 1063-4584 |
| Popis: | Objective: To identify the CD44-receptor-mediated effects of 5–7×105MW hyaluronan (HA, Hyalgan®) on cell viability in normal and damaged human chondrocyte primary cultures isolated from articular cartilage.Design: Primary cultures of human chondrocytes were established from normal articular biopsies and expanded to the second culture passage. The dose–response effects of HA on the viability of normal cultures were identified. Chondrocytes were then treated with either hypoxanthine (2mM) and xanthine oxidase (20–60mU), or with activated polymorphonuclear leukocytes (PMNs) to induce injury. Damaged and control cells were then treated with 5–7×105HA in the previously identified optimal dose of 0.05mg/ml. Viability was assessed at specific time periods for the chemically and PMN-damaged cells. To identify if HA effects were mediated by the CD44 receptor, chondrocytes were incubated with anti-CD44 antibody at saturating concentrations (5μg/ml for 100000 cells) to produce a maximum inhibition of HA binding. Cells were evaluated using the MTT viability assay, histology, electron microscopy and immunohistochemistry.Results: Direct addition of HA (optimal dose, 0.5mg/ml) significantly increased cell survival in normal chondrocyte primary cultures (P |
| Databáze: | OpenAIRE |
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