Characterization of structure and mechanism of transfection-active peptide–DNA complexes
| Autor: | Sergei Zaitsev, Marc Schneider, Roman Dallüge, Annekathrin Haberland, Michael Böttger, Gleb B. Sukhorukov, Heidi Zastrow |
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| Rok vydání: | 2002 |
| Předmět: |
Circular dichroism
Molecular Conformation Biophysics Peptide Sodium Chloride Microscopy Atomic Force Transfection Biochemistry Cell Line chemistry.chemical_compound Dynamic light scattering Structural Biology Genetics Humans A-DNA Particle Size Luciferases chemistry.chemical_classification Chemistry Circular Dichroism Chloroquine DNA Ligand (biochemistry) Molecular Weight Crystallography Particle size Peptides |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1576:45-52 |
| ISSN: | 0167-4781 |
| Popis: | We studied a number of physicochemical parameters of transfection-active peptide-DNA complexes including size, aggregation behaviour and circular dichroism (CD) spectra. These data were brought in relationship to the transfection activity of these peptides in order to better understand the mechanism of peptide-mediated gene transfer. A DNA binding oligolysine (K(16)) and a peptide comprising K(16) with an added peptide loop containing the arbitrary sequence RAD not known as a receptor ligand were used. Whereas the K(16)-DNA complex at 88% charge neutralization of the DNA phosphates collapsed into small toroidal particles with a diameter of 200 nm by dynamic light scattering, K(16)-cRAD did not. Instead, large aggregates were observed. CD spectra showed that the K(16)-DNA complexes were in a -psi state observed at liquid crystalline phases. Increasing positive charge by addition of further K(16) or disturbing the -psi state by introducing the RAD-peptide loop resulted in increasing instability indicated by aggregation and loss of the -psi CD spectrum of the complexes. Transfection experiments indicated that the aggregated material was the transfection-active component. |
| Databáze: | OpenAIRE |
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