Saccharide dosage content of meningococcal polysaccharide conjugate vaccines determined using WHO International Standards for serogroup A, C, W, Y and X polysaccharides
| Autor: | Nicola J. Beresford, Fang Gao, Kay Lockyer, Peter Rigsby, Barbara Bolgiano, Karena Burkin |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Meningococcal Vaccines Bioengineering Serogroup World Health Organization Polysaccharide Applied Microbiology and Biotechnology MENINGOCOCCAL POLYSACCHARIDE 03 medical and health sciences Immunogenicity Vaccine 0302 clinical medicine Conjugate vaccine Potency 030212 general & internal medicine Food science Vaccine Potency Pharmacology chemistry.chemical_classification Vaccines Conjugate General Immunology and Microbiology Immunogenicity Polysaccharides Bacterial General Medicine Antibodies Bacterial Meningococcal Infections 030104 developmental biology chemistry MENINGOCOCCAL POLYSACCHARIDE CONJUGATE Biotechnology Conjugate |
| Zdroj: | Biologicals. 70:53-58 |
| ISSN: | 1045-1056 |
| DOI: | 10.1016/j.biologicals.2021.01.001 |
| Popis: | Potency of meningococcal polysaccharide-protein conjugate vaccines relies on the polysaccharide content to prevent meningitis. NIBSC, as the official national control laboratory in UK, analysed ten different mono- and multi-meningococcal conjugate vaccines, using established International Standards for meningococcal serogroups A, C, W, Y and X, by resorcinol or HPAEC-PAD assay. Most saccharide contents were within ±20% of their claimed content for licensure with taking different O-acetylation levels into consideration, with only MenC content in two vaccines below (by 60% and 54%) the labelled value, however, previous study showed different dosage was not necessarily correlated to the immunogenicity of those vaccines. This study demonstrated the use of International Standards to quantify saccharide content in polysaccharide-based vaccines with different percentage of O-acetylation. These International Standards are suitable to serve as either quantitative standard or calibrator of in-house standards, with supplied stability data. |
| Databáze: | OpenAIRE |
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