Peroxiredoxins are required for spindle assembly, chromosome organization, and polarization in mouse oocytes
| Autor: | Dong-Hyung Cho, Eunji Kim, Sang-Young Chun, Jeong Su Oh, Yong Seok Park, Jae-Sung Kim, Hyuk-Joon Jeon, Ho Zoon Chae |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA damage Biophysics Cortical granule Spindle Apparatus Biology Biochemistry Mice Structure-Activity Relationship 03 medical and health sciences Meiosis Quinoxalines medicine Animals Molecular Biology Cells Cultured Actin Dose-Response Relationship Drug 030102 biochemistry & molecular biology Cell Polarity Microtubule organizing center Peroxiredoxins Cell Biology Oocyte Chromosomes Mammalian Cell biology 030104 developmental biology medicine.anatomical_structure Oocytes Female Peroxiredoxin Multipolar spindles |
| Zdroj: | Biochemical and Biophysical Research Communications. 489:193-199 |
| ISSN: | 0006-291X |
| Popis: | Peroxiredoxins (Prxs) are highly conserved antioxidant enzymes and are implicated in multiple biological processes; however, their function in oocyte meiosis has not been studied. Here we show that inhibition of Prx I and II results in spindle defects, chromosome disorganization, and impaired polarization in mouse oocytes. Prx I was specifically localized at the spindle, whereas Prx II was enriched at the oocyte cortex and chromosomes. Inhibition of Prx activity with conoidin A disturbed assembly of the microtubule organizing center (MTOC) through Aurora A regulation, leading to defects in spindle formation. Moreover, conoidin A impaired actin filament and cortical granule (CG) distribution, disrupting actin cap and CG formation, respectively. Conoidin A also increased DNA damage without significantly increasing reactive oxygen species (ROS) levels, suggesting that the effects of conoidin A on meiotic maturation are not likely associated with ROS scavenging pathways. Therefore, our data suggest that Prxs are required for spindle assembly, chromosome organization, and polarization during meiotic maturation. |
| Databáze: | OpenAIRE |
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