Omega-3 polyunsaturated fatty acids attenuate inflammatory activation and alter differentiation in human adipocytes
| Autor: | Rachana Shah, Yasmeen Midgette, Kailey Roberts-Lee, Jane F. Ferguson, Holly M. Smith, Cristina Borcea |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides medicine.medical_specialty Docosahexaenoic Acids Endocrinology Diabetes and Metabolism Clinical Biochemistry Adipose tissue 030204 cardiovascular system & hematology Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Adipocyte Fatty Acids Omega-3 medicine Adipocytes Leukocytes Humans Adiponectin secretion Obesity Omega 3 fatty acid Molecular Biology Cells Cultured chemistry.chemical_classification Inflammation Nutrition and Dietetics Macrophages Cell Differentiation Lipid Droplets Eicosapentaenoic acid Coculture Techniques 030104 developmental biology Endocrinology chemistry Eicosapentaenoic Acid Docosahexaenoic acid Adipogenesis lipids (amino acids peptides and proteins) Polyunsaturated fatty acid |
| Popis: | Background: Omega-3 polyunsaturated fatty acids, specifically the fish-oil-derived eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been proposed as inflammation-resolving agents via their effects on adipose tissue. Objective: We proposed to determine the effects of EPA and DHA on human adipocyte differentiation and inflammatory activation in vitro. Methods: Primary human subcutaneous adipocytes from lean and obese subjects were treated with 100 μM EPA and/or DHA throughout differentiation (differentiation studies) or for 72 h postdifferentiation (inflammatory studies). THP-1 monocytes were added to adipocyte wells for co-culture experiments. Subcutaneous and visceral adipose explants from obese subjects were treated for 72 h with EPA and DHA. Oil Red O staining was performed on live cells. Cells were collected for mRNA analysis by quantitative polymerase chain reaction, and media were collected for protein quantification by enzyme-linked immunosorbent assay. Results: Incubation with EPA and/or DHA attenuated inflammatory response to lipopolysaccharide (LPS) and monocyte co-culture with reduction in post-LPS mRNA expression and protein levels of IL6, CCL2 and CX3CL1. Expression of inflammatory genes was also reduced in the endogenous inflammatory response in obese adipose. Both DHA and EPA reduced lipid droplet formation and lipogenic gene expression without alteration in expression of adipogenic genes or adiponectin secretion. Conclusions: EPA and DHA attenuate inflammatory activation of in vitro human adipocytes and reduce lipogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|