Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress
| Autor: | Nong Qian, Qiang Liang, Wang Weizan, Liu Xiaoyan, Xie Jinlan, Manoj Kumar Solanki, Li-Tao Yang, Yang-Rui Li, Li Yijie, Chang-Ning Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Drought tolerance Biology 01 natural sciences Homology (biology) Article 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Gene expression Botany Gene Plant Proteins Genetics Regulation of gene expression Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Gene Expression Regulation Developmental Water Droughts Saccharum Gene expression profiling Plant Leaves 030104 developmental biology Gene Ontology Gene chip analysis Signal transduction 010606 plant biology & botany Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep25698 |
| Popis: | Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate and severe) during the elongation stage and gene expression was analyzed using microarray technology. Physiological parameters of sugarcane showed significant alterations in response to drought stress. Based on the expression profile of 15,593 sugarcane genes, 1,501 (9.6%) genes were differentially expressed under different water-level treatments; 821 genes were upregulated and 680 genes were downregulated. A gene similarity analysis showed that approximately 62.6% of the differentially expressed genes shared homology with functional proteins. In a Gene Ontology (GO) analysis, 901 differentially expressed genes were assigned to 36 GO categories. Moreover, 325 differentially expressed genes were classified into 101 pathway categories involved in various processes, such as the biosynthesis of secondary metabolites, ribosomes, carbon metabolism, etc. In addition, some unannotated genes were detected; these may provide a basis for studies of water-deficit tolerance. The reliability of the observed expression patterns was confirmed by RT-PCR. The results of this study may help identify useful genes for improving drought tolerance in sugarcane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|