PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness
| Autor: | Nanpeng Li, Chunxing Yang, Changwei Lin, Lihua Huang, Runliu Wu, Shenglei Song, Miao Chen, Arad Jain, Yi Zhang, Peicheng Yan, Zhicai Feng, Yang Bai, Chen Su, Xiaorong Li, Daojiang Li, Yuheng Du |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Research paper Colorectal cancer Carcinogenesis Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Cancer stem cell Cell Line Tumor medicine Animals Humans Promoter Regions Genetic Gene 3' Untranslated Regions Cell Proliferation POFUT1 Oncogene Mechanism (biology) RNA-Binding Proteins General Medicine medicine.disease Fucosyltransferases Xenograft Model Antitumor Assays Co-expression DNA-Binding Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Bidirectional promoter 030220 oncology & carcinogenesis Cancer research Neoplastic Stem Cells Ectopic expression PLAGL2 Stem cell Colorectal Neoplasms Transcription Factors |
| Zdroj: | EBioMedicine |
| ISSN: | 2352-3964 |
| Popis: | Background Our previous study revealed that PLAGL2 or POFUT1 can promote tumorigenesis and maintain significant positive correlations in colorectal cancer (CRC). However, the mechanism leading to the co-expression and the underlying functional and biological implications remain unclear. Methods Clinical tumor tissues and TCGA dataset were utilized to analyze the co-expression of PLAGL2 and POFUT1. Luciferase reporter assays, specially made bidirectional promoter vectors and ectopic expression of 3’UTR were employed to study the mechanisms of co-expression. In vitro and in vivo assays were performed to further confirm the oncogenic function of both. The sphere formation assay, immunofluorescence, Western blot and qRT-PCR were performed to investigate the effect of both genes in colorectal cancer stem cells (CSCs). Findings PLAGL2 and POFUT1 maintained co-expression in CRC (r = 0.91, p |
| Databáze: | OpenAIRE |
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