PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness

Autor: Nanpeng Li, Chunxing Yang, Changwei Lin, Lihua Huang, Runliu Wu, Shenglei Song, Miao Chen, Arad Jain, Yi Zhang, Peicheng Yan, Zhicai Feng, Yang Bai, Chen Su, Xiaorong Li, Daojiang Li, Yuheng Du
Rok vydání: 2019
Předmět:
Zdroj: EBioMedicine
ISSN: 2352-3964
Popis: Background Our previous study revealed that PLAGL2 or POFUT1 can promote tumorigenesis and maintain significant positive correlations in colorectal cancer (CRC). However, the mechanism leading to the co-expression and the underlying functional and biological implications remain unclear. Methods Clinical tumor tissues and TCGA dataset were utilized to analyze the co-expression of PLAGL2 and POFUT1. Luciferase reporter assays, specially made bidirectional promoter vectors and ectopic expression of 3’UTR were employed to study the mechanisms of co-expression. In vitro and in vivo assays were performed to further confirm the oncogenic function of both. The sphere formation assay, immunofluorescence, Western blot and qRT-PCR were performed to investigate the effect of both genes in colorectal cancer stem cells (CSCs). Findings PLAGL2 and POFUT1 maintained co-expression in CRC (r = 0.91, p
Databáze: OpenAIRE