Ribonucleotide Sequence Homology Among Avian Oncornaviruses
| Autor: | M. A. Baluda, M. Shoyab |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
animal structures
viruses Immunology RNA-dependent RNA polymerase Bone Marrow Cells Biology Microbiology Birds chemistry.chemical_compound Bone Marrow Transcription (biology) Culture Techniques Virology Animal Viruses RNA polymerase I Animals Avian Myeloblastosis Virus Avian Leukosis Virus Base Sequence Nucleic Acid Hybridization RNA Nuclease protection assay DNA DNA Neoplasm Ribonucleotides Non-coding RNA Molecular biology Avian Leukosis Avian Sarcoma Viruses chemistry RNA editing Insect Science embryonic structures Nucleic Acid Conformation RNA Viral Chickens |
| Zdroj: | Journal of Virology. 17:106-113 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | RNA sequence relatedness among avian RNA tumor virus genomes was analyzed by inhibition of DNA-RNA hybrid formation between 3 H-labeled 35 S viral RNA and an excess of leukemic or normal chicken cell DNA with increasing concentrations of unlabeled 35 S viral RNA. The avian viruses tested were Rous associated virus (RAV)-0, avian myeloblastosis virus (AMV), RAV-60, RAV-61, and B-77 sarcoma virus. Hybridization of 3 H-labeled 35 S AMV RNA with DNA from normal chicken cells was inhibited by unlabeled 35 S RAV-0 RNA as efficiently (100%) as by unlabeled AMV RNA. Hybridization between 3 H-labeled 35 S AMV RNA and DNA from leukemic chicken myeloblasts induced by AMV was suppressed 100 and 68% by unlabeled 35 S RNA from AMV and RAV-0, respectively. Hybridization between 3 H-labeled RAV-0 and leukemic chicken myeloblast DNA was inhibited 100 and 67% by unlabeled 35 S RNA from RAV-0 and AMV, respectively. It appears therefore that the AMV and RAV-0 genomes are 67 to 70% homologous and that AMV hybridizes to RAV-0 like sequences in normal chicken DNA. Hybridization between AMV RNA and leukemic chicken DNA was inhibited 40% by RNA from RAV-60 or RAV-61 and 50% by B-77 RNA. Hybridization between RAV-0 RNA and leukemic chicken DNA was inhibited 80% by RAV-60 or RAV-61 and 70% by B-77 RNA. Hybridization between 3 H-labeled 35 S RNA from RAV-60 or RAV-61 and leukemic chicken myeloblast DNA was reduced equally by RNA from RAV-60, RAV-61, AMV or RAV-0; this suggests that RNA from RAV-60 and RAV-61 hybridizes with virus-specific sequences in leukemic DNA which are shared by AMV, RAV-0, RAV-60, and RAV-61 RNAs. Hybridization between 3 H-labeled 35 S RNA from RAV-61 and normal pheasant DNA was inhibited 100% by homologous viral RNA, 22 to 26% by RNA from AMV or RAV-0, and 30 to 33% by RNA from RAV-60 or B-77. Nearly complete inhibition of hybridization between RAV-0 RNA and leukemic chicken DNA by a mixture of AMV and B-77 35 S RNAs indicates that the RNA sequences shared by B-77 virus and RAV-0 are different from the sequences shared by AMV and RAV-0. It appears that different avian RNA tumor virus genomes have from 50 to 80% homology in nucleotide sequences and that the degree of hybridization between normal chicken cell DNA and a given viral RNA can be predicted from the homology that exists between the viral RNA tested and RAV-0 RNA. |
| Databáze: | OpenAIRE |
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