AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity

Autor: Margaret Lin Veruki, Bas-Jan Zandt, Eirik Madsen, Espen Hartveit, Svein Harald Mørkve, Aurea F. Castilho
Rok vydání: 2017
Předmět:
0301 basic medicine
Histology
Patch-Clamp Techniques
Action Potentials
Glutamic Acid
AMPA receptor
Biology
Neurotransmission
Ribbon synapse
In Vitro Techniques
Synaptic Transmission
Retina
Amacrine cells
03 medical and health sciences
0302 clinical medicine
Postsynaptic potential
medicine
Image Processing
Computer-Assisted
Animals
Kinetic scheme
Receptors
AMPA
Long-term depression
Neurons
Stochastic Processes
Microscopy
Confocal
Dose-Response Relationship
Drug
General Neuroscience
Glutamate receptors
Phosphoproteins
Electric Stimulation
Rats
DNA-Binding Proteins
Alcohol Oxidoreductases
Kinetics
030104 developmental biology
medicine.anatomical_structure
Silent synapse
Synapses
Female
sense organs
Synaptic signaling
Patch clamp
Anatomy
Neuroscience
Co-Repressor Proteins
Disks Large Homolog 4 Protein
030217 neurology & neurosurgery
Zdroj: Brain Structure and Function
769–804
ISSN: 1863-2661
Popis: In chemical synapses, neurotransmitter molecules released from presynaptic vesicles activate populations of postsynaptic receptors that vary in functional properties depending on their subunit composition. Differential expression and localization of specific receptor subunits are thought to play fundamental roles in signal processing, but our understanding of how that expression is adapted to the signal processing in individual synapses and microcircuits is limited. At ribbon synapses, glutamate release is independent of action potentials and characterized by a high and rapidly changing rate of release. Adequately translating such presynaptic signals into postsynaptic electrical signals poses a considerable challenge for the receptor channels in these synapses. Here, we investigated the functional properties of AMPA receptors of AII amacrine cells in rat retina that receive input at spatially segregated ribbon synapses from OFF-cone and rod bipolar cells. Using patch-clamp recording from outside-out patches, we measured the concentration dependence of response amplitude and steady-state desensitization, the single-channel conductance and the maximum open probability. The GluA4 subunit seems critical for the functional properties of AMPA receptors in AII amacrines and immunocytochemical labeling suggested that GluA4 is located at synapses made by both OFF-cone bipolar cells and rod bipolar cells. Finally, we used a series of experimental observables to develop kinetic models for AII amacrine AMPA receptors and subsequently used the models to explore the behavior of the receptors and responses generated by glutamate concentration profiles mimicking those occurring in synapses. These models will facilitate future in silico modeling of synaptic signaling and processing in AII amacrine cells. acceptedVersion
Databáze: OpenAIRE
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