Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients
| Autor: | Bernard A. Zonnenberg, Joost S.P. Vermaat, Niven Mehra, M. F. G. B. Gebbink, Emile E. Voest, Laurens V. Beerepoot |
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| Rok vydání: | 2004 |
| Předmět: |
Vascular Endothelial Growth Factor A
Placental growth factor CD31 Pathology medicine.medical_specialty Angiogenesis Fluorescent Antibody Technique Angiogenesis Inhibitors Stem cell factor Endothelial Growth Factors Neoplasms von Willebrand Factor Biomarkers Tumor Humans Medicine Neovascularization Pathologic business.industry Endothelial Cells Cancer Hematology medicine.disease Endothelial stem cell medicine.anatomical_structure Oncology Disease Progression cardiovascular system Cytokines CD146 Endothelium Vascular Bone marrow business Follow-Up Studies |
| Zdroj: | Annals of Oncology. 15:139-145 |
| ISSN: | 0923-7534 |
| DOI: | 10.1093/annonc/mdh017 |
| Popis: | BACKGROUND There is accumulating evidence from preclinical studies that circulating endothelial cells (CECs) play an important role in neovascularization and tumor growth. The role of CECs in human cancer progression is sparsely investigated. We therefore analyzed CECs in peripheral blood of cancer patients. In addition, we correlated CEC levels in these patients with plasma levels of cytokines that are known to mobilize CECs in experimental models. PATIENTS AND METHODS Viable CECs were isolated, quantified and cultured from cancer patients' whole blood by using magnetic beads coupled to an antibody directed against CD146, a pan-endothelial marker. Viable cells were visualized by calceinAM staining. Positive staining for specific endothelial cell markers [i.e. von Willebrand factor, CD31, vascular endothelial cell growth factor (VEGF) receptor-2] was used to confirm the endothelial phenotype. RESULTS Cancer patients with progressive disease (95 patients) had on average 3.6-fold more CECs than healthy subjects (46 patients, P |
| Databáze: | OpenAIRE |
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