Bleomycin-induced genotoxicity in vitro in human peripheral blood lymphocytes evidenced as complex chromosome- and chromatid-type aberrations
| Autor: | Aswathy S. Lal, Ravi Maddaly, S. Kauser Begum, Sraddha S Bharadwaj, Solomon F.D. Paul, V Lalitha, J. Vijayalakshmi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Mitotic index Chromatids Toxicology medicine.disease_cause Bleomycin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Mitotic Index medicine Humans Lymphocytes Cells Cultured Chromosome Aberrations Antibiotics Antineoplastic Chromosome General Medicine Molecular biology In vitro Tolerance induction 030104 developmental biology chemistry 030220 oncology & carcinogenesis Chromatid Genotoxicity DNA Mutagens |
| Zdroj: | Toxicology in Vitro. 54:367-374 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2018.10.013 |
| Popis: | Bleomycin is a chemotherapeutic and a radiomimetic drug which induces single and double-strand breaks in DNA by forming free radicals. We demonstrate in this study the capacity of bleomycin in inducing complex chromosome- and chromatid-type aberrations. Human peripheral blood was exposed to different concentrations of bleomycin (0, 10, 20, 30 and 40 μg/mL) and the aberrations induced were studied. The chromosomal-type aberrations studied were dicentrics, tricentrics, tetracentrics, centric rings and acentric fragments. The chromatid-type aberrations studied were double minutes, terminal lesions and terminal deletions. Though the overall trends that we obtained in the dose-dependent mitotic index and the chromosome- and chromatid-type aberrations conform to the reported literature, we could observe enhanced numbers and the types of such damages in this study. We could notice that chromosome-type aberrations were more than the chromatid-type aberrations. The enhanced numbers and the types of aberrations induced pave way for enhancing the sensitivity of genotoxic assays. Also, with more numbers and type of aberrations available, it would be useful to study the mechanisms of genotoxicity of drugs and in understanding phenomena such as “tolerance induction” to chronic exposure to such mutagens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect