Hemolytic, anticancer and antigiardial activity of Palythoa caribaeorum venom
| Autor: | Alejandro García-Arredondo, Saúl Gómez-Manzo, Fernando Lazcano-Pérez, Barbarín Arreguín-Lozano, Yadira Rufino-González, Martha Ponce-Macotela, Roberto Arreguín-Espinosa, Miguel Cuevas-Cruz, Ariana Zavala-Moreno, Jaime Marcial-Quino |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Palythoa caribaeorum Giardiasis lcsh:Arctic medicine. Tropical medicine Antiparasitic medicine.drug_class lcsh:RC955-962 Venom Pharmacology Cnidarian Toxicology 03 medical and health sciences lcsh:RA1190-1270 lcsh:Zoology medicine lcsh:QL1-991 Cytotoxicity lcsh:Toxicology. Poisons 030102 biochemistry & molecular biology Chemistry Research medicine.disease Hemolysis In vitro Infectious Diseases Antitumoral effect Toxicity Cytotoxin Adenocarcinoma Animal Science and Zoology Parasitology K562 cells |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-7 (2018) Journal of Venomous Animals and Toxins including Tropical Diseases v.24 2018 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP The Journal of Venomous Animals and Toxins Including Tropical Diseases Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 24, Article number: 12, Published: 24 MAY 2018 |
| ISSN: | 1678-9199 |
| DOI: | 10.1186/s40409-018-0149-8 |
| Popis: | Background Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis. Methods The presence of cytolysins and vasoconstrictor activity of P. caribaeorum venom were determined by hemolysis, PLA2 and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), K562 (human chronic myelogenous leukemia), U251 (human glyoblastoma), PC-3 (human prostatic adenocarcinoma) and SKLU-1 (human lung adenocarcinoma). An in vivo toxicity assay was performed with crickets and the antiparasitic assay was performed against G. intestinalis at 24 h of incubation. Results P. caribaeorum venom produced hemolytic and PLA2 activity and showed specific cytotoxicity against U251 and SKLU-1 cell lines, with approximately 50% growing inhibition. The venom was toxic to insects and showed activity against G. intestinalis in a dose-dependent manner by possibly altering its membrane osmotic equilibrium. Conclusion These results suggest that P. caribaeorum venom contains compounds with potential therapeutic value against microorganisms and cancer. |
| Databáze: | OpenAIRE |
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