Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients
| Autor: | Ismael Dale C. G. Silva, Bruno Rafael Müller, Alfredo Gragnani, Lydia Masako Ferreira, Samuel Marcos Ribeiro de Noronha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Keratinocytes Pathology medicine.medical_specialty Time Factors Fibroblast Growth Factor 7 RD1-811 Interleukin-1beta Gene Expression Polymerase Chain Reaction chemistry.chemical_compound Gene expression medicine Humans Beta (finance) Cells Cultured Skin Messenger RNA business.industry Tumor Necrosis Factor-alpha Interleukin Fibroblasts Molecular biology chemistry Cell culture Case-Control Studies Tumor necrosis factor alpha Surgery Female Keratinocyte growth factor business Burns Interleukin-1 |
| Zdroj: | Acta Cirúrgica Brasileira v.28 n.8 2013 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 28, Issue: 8, Pages: 551-558, Published: AUG 2013 Acta Cirurgica Brasileira, Vol 28, Iss 8, Pp 551-558 (2013) |
| Popis: | PURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients. METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta. RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p |
| Databáze: | OpenAIRE |
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