Novobiocin forms cation-permeable ion channels in rat fetal distal lung epithelium
| Autor: | Canhui Li, C. Bear, Bijan Rafii, J. Correa, Hugh O'Brodovich, Xiaodong Wang |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Physiology
Lipid Bilayers Biology Epithelium Ion Channels Permeability Fetus Cations medicine Extracellular Animals Lipid bilayer Lung Ion transporter Novobiocin Ion channel Epithelial Cells Cell Biology Membrane transport Apical membrane Rats Electrophysiology carbohydrates (lipids) Biochemistry Biophysics Calcium Intracellular medicine.drug |
| Zdroj: | American Journal of Physiology-Cell Physiology. 264:C1532-C1537 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1993.264.6.c1532 |
| Popis: | The antibiotic novobiocin has been previously reported to increase Na+ transport in frog skin, presumably by attenuation of Na+ self-inhibition of Na+ channels. To determine whether novobiocin had similar effects and utilized a similar mechanism in mammalian Na(+)-transporting tissues, we studied its effect on ion transport by primary cultures of fetal distal lung epithelium (FDLE) cultured from 20-day gestationally aged rats (term = 22 days). Novobiocin (10 mM) increased short-circuit current and markedly decreased the resistance in FDLE monolayers mounted in Ussing chambers. Fura-2 single-cell studies showed that novobiocin increased intracellular Ca2+ concentration and that this resulted from extracellular sources. Nystatin-perforated patch-clamp techniques demonstrated that novobiocin increased nonrectifying cation whole cell currents without inducing detectable anion currents. Novobiocin created nonrectifying monovalent cation-selective channels in lipid bilayers. These studies demonstrated that novobiocin affects the bioelectric properties of Na+ transporting lung epithelium and that this likely occurs by the formation of ion-permeant channels in their lipid membranes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|